Sasaki H, Takabayashi T, Ozawa N, Sasamoto K, Shintaku Y, Yajima A
Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai.
Nihon Sanka Fujinka Gakkai Zasshi. 1988 Jul;40(7):903-8.
Chromosomes, which can be seen under a light microscope (LM) as bands, are too thin to see with a scanning electron microscope (SEM). We noticed that chromosomes increase in thickness with Giemsa staining, so that scanning microscopic specimens could be prepared by long-time Giemsa staining for SEM observation, and in the present study the following results were obtained. 1) When the duration of the trypsin treatment was kept constant, and the Giemsa staining was performed from 30-min to 24-h, it was found that the thickness of the chromosomes increased with the length of treatment time, and the fibrous structures were clearly seen. 2) While a sufficient thickness of the chromosomes was obtained with the long-time Giemsa staining, the bands became unclear as the time of staining was extended. 3) A long-time Giemsa staining without trypsin treatment showed more "highly packed" fibers, so that the trypsin treatment was felt to be related to the dispersion of the fibers.
染色体在光学显微镜（LM）下可呈现为条带，但细得无法用扫描电子显微镜（SEM）观察到。我们注意到经吉姆萨染色后染色体的厚度会增加，这样就可以通过长时间吉姆萨染色制备扫描显微镜标本用于SEM观察，在本研究中获得了以下结果。1）当胰蛋白酶处理时间保持恒定时，进行30分钟至24小时的吉姆萨染色，发现染色体厚度随处理时间延长而增加，并且纤维结构清晰可见。2）虽然长时间吉姆萨染色可使染色体获得足够厚度，但随着染色时间延长条带变得模糊不清。3）未经胰蛋白酶处理的长时间吉姆萨染色显示出更多“高度紧密排列”的纤维，因此认为胰蛋白酶处理与纤维的分散有关。
