Qu Z X, Fertel R, Neff N H, Hadjiconstantinou M
Department of Pharmacology, Ohio State University College of Medicine, Columbus.
J Pharmacol Exp Ther. 1988 Sep;246(3):839-42.
Incubation of the rat retina with acetylcholine resulted in about a 20 to 30% decrease of basal cyclic AMP accumulation. Oxotremorine, arecoline, [4-hydroxy-2-butynyl]trimethylammonium chloride, m-chlorocarbanilate and carbachol also inhibited cyclic AMP accumulation. Nicotine had no effect. The response was blocked by atropine and pirenzepine but not gallamine. Intraocular injection of pertussis toxin 72 hr before testing also blocked the response to acetylcholine. The presence of forskolin exaggerated the response to acetylcholine. Intraocular injection of the cholinotoxin AF64A resulted in apparent supersensitivity of the response to acetylcholine. Our results suggest that rat retina contains muscarinic M1 receptors that are coupled negatively to adenylate cyclase.
用乙酰胆碱孵育大鼠视网膜会导致基础环磷酸腺苷（cAMP）积累减少约20%至30%。氧化震颤素、槟榔碱、[4-羟基-2-丁炔基]三甲基氯化铵、间氯卡班酸酯和卡巴胆碱也会抑制cAMP积累。尼古丁没有作用。该反应被阿托品和哌仑西平阻断，但未被加拉明阻断。在测试前72小时眼内注射百日咳毒素也会阻断对乙酰胆碱的反应。福斯高林的存在会增强对乙酰胆碱的反应。眼内注射胆碱毒素AF64A会导致对乙酰胆碱的反应明显超敏。我们的结果表明，大鼠视网膜含有与腺苷酸环化酶负偶联的毒蕈碱M1受体。
