抵抗素-420C>G启动子变异与结直肠癌风险

Resistin -420C>G promoter variant and colorectal cancer risk.

作者信息

Mahmoudi Touraj, Karimi Khatoon, Arkani Maral, Farahani Hamid, Vahedi Mohsen, Dabiri Reza, Nobakht Hossein, Asadi Asadollah, Mirakhorli Mojgan, Arshi Benafsheh, Derakhshan Arash, Zali Mohammad Reza

机构信息

Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran - Iran.

出版信息

Int J Biol Markers. 2014 Sep 30;29(3):e233-8. doi: 10.5301/jbm.5000079.

DOI:10.5301/jbm.5000079

PMID:24585435

Abstract

PURPOSES

Obesity is associated with an increased risk of colorectal cancer (CRC), and ghrelin (GHRL) and resistin (RETN) are thought to be related to obesity. Our aim was to investigate whether GHRL and RETN gene variants are associated with CRC risk.

MATERIALS AND METHODS

All 414 subjects, including 197 cases with CRC and 217 controls, were genotyped for the GHRL (rs26802) and RETN (rs1862513) or -420 C>G gene variants using the PCR-RFLP method.

RESULTS

Our findings indicated that the RETN -420 C>G "CC" genotype, compared with the "GG" and "GC" genotypes, was a marker of decreased CRC susceptibility; the difference remained significant after adjustment for age, BMI, gender, smoking status, NSAID use, and family history of CRC (p=0.020; OR=0.52, 95% CI=0.30-0.90). Furthermore, after adjustment for confounding factors, the -420 C>G "CC" genotype, compared with the "GG" genotype, was associated with a decreased risk for CRC (p=0.044; OR=0.53, 95% CI=0.29-0.98). In addition, no significant difference was observed for the GHRL (rs26802) gene variant.

CONCLUSIONS

To our knowledge, this is the first study suggesting that the RETN -420 C>G "CC" genotype is a marker of decreased CRC susceptibility. This observation is relevant from a scientific perspective and deserves further investigations.

摘要

目的

肥胖与结直肠癌（CRC）风险增加相关，而胃饥饿素（GHRL）和抵抗素（RETN）被认为与肥胖有关。我们的目的是研究GHRL和RETN基因变异是否与CRC风险相关。

材料与方法

使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，对414名受试者（包括197例CRC患者和217名对照）的GHRL（rs26802）和RETN（rs1862513）或-420 C>G基因变异进行基因分型。

结果

我们的研究结果表明，与“GG”和“GC”基因型相比，RETN -420 C>G“CC”基因型是CRC易感性降低的一个标志物；在调整年龄、体重指数、性别、吸烟状况、非甾体抗炎药使用情况和CRC家族史后，差异仍然显著（p=0.020；比值比[OR]=0.52，95%置信区间[CI]=0.30-0.90）。此外，在调整混杂因素后，与“GG”基因型相比，-420 C>G“CC”基因型与CRC风险降低相关（p=0.044；OR=0.53，95% CI=0.29-0.98）。此外，未观察到GHRL（rs26802）基因变异有显著差异。