De Smet Lina, De Koker Dieter, Hawley Alyse K, Foster Leonard J, De Vos Paul, de Graaf Dirk C
Ghent University, Laboratory of Zoophysiology, Department of Physiology, Ghent, Belgium.
University of British Columbia, Department of Microbiology & Immunology, Vancouver, Canada.
PLoS One. 2014 Feb 20;9(2):e89175. doi: 10.1371/journal.pone.0089175. eCollection 2014.
Paenibacillus larvae, the causal agent of American Foulbrood disease (AFB), affects honey bee health worldwide. The present study investigates the effect of bodily fluids from honey bee larvae on growth velocity and transcription for this Gram-positive, endospore-forming bacterium. It was observed that larval fluids accelerate the growth and lead to higher bacterial densities during stationary phase. The genome-wide transcriptional response of in vitro cultures of P. larvae to larval fluids was studied by microarray technology. Early responses of P. larvae to larval fluids are characterized by a general down-regulation of oligopeptide and sugar transporter genes, as well as by amino acid and carbohydrate metabolic genes, among others. Late responses are dominated by general down-regulation of sporulation genes and up-regulation of phage-related genes. A theoretical mechanism of carbon catabolite repression is discussed.
幼虫芽孢杆菌是美洲幼虫腐臭病（AFB）的病原体，在全球范围内影响蜜蜂健康。本研究调查了蜜蜂幼虫体液对这种革兰氏阳性、形成芽孢细菌的生长速度和转录的影响。观察到幼虫体液可加速生长，并在稳定期导致更高的细菌密度。通过微阵列技术研究了幼虫芽孢杆菌体外培养物对幼虫体液的全基因组转录反应。幼虫芽孢杆菌对幼虫体液的早期反应特征是寡肽和糖转运蛋白基因以及氨基酸和碳水化合物代谢基因等普遍下调。晚期反应主要是芽孢形成基因普遍下调和噬菌体相关基因上调。文中讨论了碳分解代谢物阻遏的理论机制。
