兔血管紧张素转换酶cDNA克隆的分离：肺和睾丸同工酶两种不同mRNA的鉴定。
Isolation of cDNA clones of rabbit angiotensin converting enzyme: identification of two distinct mRNAs for the pulmonary and the testicular isozymes.
作者信息
Roy S N, Kusari J, Soffer R L, Lai C Y, Sen G C
机构信息
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, N.Y. 10021.
出版信息
Biochem Biophys Res Commun. 1988 Sep 15;155(2):678-84. doi: 10.1016/s0006-291x(88)80548-5.
We have isolated cDNA clones of rabbit angiotensin converting enzyme. These clones were isolated by antibody-screening of a lambda gt11 expression library made from rabbit testicular mRNA. The 2.6 kb insert of one such clone was subcloned in pBR322 and used as a hybridization probe. Out of the twenty independently isolated clones only seven hybridized with this probe suggesting that these clones belong to at least two families. Northern analysis revealed the presence of a 2.6 kb mRNA in rabbit testes and a 5.0 kb mRNA in rabbit lungs which hybridized strongly with this probe. These results indicate that the two tissue-specific isozymic forms of angiotensin converting enzyme are encoded by two distinct mRNAs which share sequence homologies.
我们已经分离出了兔血管紧张素转换酶的cDNA克隆。这些克隆是通过对用兔睾丸mRNA构建的λgt11表达文库进行抗体筛选而分离得到的。将其中一个这样的克隆的2.6kb插入片段亚克隆到pBR322中，并用作杂交探针。在20个独立分离的克隆中，只有7个与该探针杂交，这表明这些克隆至少属于两个家族。Northern分析显示，兔睾丸中存在一个2.6kb的mRNA，兔肺中存在一个5.0kb的mRNA，它们与该探针强烈杂交。这些结果表明，血管紧张素转换酶的两种组织特异性同工酶形式由两个具有序列同源性的不同mRNA编码。
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