Moskalenko A A, Toropygina O A
Nauchnye Doki Vyss Shkoly Biol Nauki. 1988(5):101-5.
A simple method of revealing the additional zones of proteins in gradient polyacrylamide gels, preliminary dyed Coomassie by means of silver ions is described. The dyeing of Coomassie allows to avoid the time-consuming stages of preliminary treatment of gels as well to reveal more sensitive zones in gels. On the second stage of dyeing silver minor zones appear there which were not seen while Coomassie was dyed. The suggested method preserves high sensitivity characteristic of the methods of gel dyeing with silver.
本文描述了一种简单的方法，可用于揭示梯度聚丙烯酰胺凝胶中蛋白质的额外区域，该凝胶预先用银离子对考马斯亮蓝进行染色。考马斯亮蓝染色可避免凝胶预处理耗时的步骤，还能揭示凝胶中更敏感的区域。在染色的第二阶段会出现一些考马斯亮蓝染色时未看到的微小银染区域。所建议的方法保留了银染凝胶方法的高灵敏度特性。
