Gal'perina T E
Biull Eksp Biol Med. 1988 Sep;106(9):350-3.
Relation between processes of proliferation and synthesis of the embryonal serum protein alpha-fetoprotein (AFP), the influence on these processes of polyelectrolyte dextran sulfate (DS) and dimethyl-sulfoxide (DMSO) has been studied in the monolayer culture of mouse hepatocytes. In control cultures the correlations between the time of appearance and the level of DNA and AFP synthesis were observed. DS and DMSO were found to inhibit both processes. Cell proliferation could be reestablished by addition of epidermal growth factor. In case of the influence of DMSO, it wasn't followed by the induction of AFP synthesis. This the processes of DNA and AFP synthesis in monolayer cultures of mouse hepatocytes can be separated. The elongated incubation of hepatocytes with collagenase during their obtaining, abolished the effects of DS. This shows that surface components of hepatocytes, lost upon enzyme degradation, may be involved in the mechanism of DS effect.
在小鼠肝细胞单层培养中，研究了胚胎血清蛋白甲胎蛋白（AFP）的增殖与合成过程之间的关系，以及聚电解质硫酸葡聚糖（DS）和二甲基亚砜（DMSO）对这些过程的影响。在对照培养中，观察到DNA和AFP合成出现的时间与水平之间的相关性。发现DS和DMSO均抑制这两个过程。添加表皮生长因子可重建细胞增殖。在DMSO的影响下，未诱导AFP合成。因此，小鼠肝细胞单层培养中DNA和AFP合成过程可以分离。在获取肝细胞时用胶原酶进行长时间孵育，消除了DS的作用。这表明肝细胞表面成分在酶降解后丢失，可能参与了DS作用的机制。
