Department of Otorhinolaryngology-Head and Neck Surgery, Hallym University College of Medicine, Seoul, Korea.
Department of Pathology, Hallym University College of Medicine, Seoul, Korea.
Clin Exp Otorhinolaryngol. 2014 Mar;7(1):53-8. doi: 10.3342/ceo.2014.7.1.53. Epub 2014 Feb 5.
To evaluate the loss of heterozygosities (LOH) of chromosomes 3p14 (FHIT gene), 9p21 (p16), 13q21 (pRb), 6q22 (E-cadherin) and 17p13 (p53) in various thyroid tumors.
Eighty thyroid tumor cases (20 follicular adenomas, 10 follicular carcinomas, and 50 papillary carcinomas) have been analyzed for the presence of LOH in chromosomes 3p14, 9p21, 13q21, 6q22, and 17p13 allelic loss, using microsatellite markers and DNA obtained from formalin-fixed paraffin-embedded archival tissues.
LOH on 3p14 was found in 10.5%, 33.3%, and 30.4% of follicular adenomas, follicular carcinomas, and papillary carcinomas, respectively. LOH on 9p21 was detected in 6%, 44.4%, and 47.8%, respectively. LOH on pRb gene was found in 5.3%, 20.0%, and 35.4%, respectively. LOH on E-cadherin gene was found in 5.3%, 22.2%, and 43.8%, respectively. LOH on 17p13 was detected in 0%, 40%, and 45.8%, respectively. LOH in FHIT gene, p16, pRb, E-cadherin, and p53 genes were more frequently identified in follicular carcinoma and papillary carcinoma than in follicular adenoma.
LOH results of the five tumor suppressor genes (FHIT gene, p16, pRb, E-cadherin, and p53) showed statistical differences between benign tumor and malignant tumor. Among papillary carcinoma, LOH in p16, E-cadherin and p53 genes well correlated with poorly differentiated grade, and LOH of E-cadherin was associated with lymph node metastasis.
评估染色体 3p14（脆性组氨酸三联体基因）、9p21（p16）、13q21（pRb）、6q22（E-钙黏蛋白）和 17p13（p53）在各种甲状腺肿瘤中的杂合性丢失（LOH）。
分析了 80 例甲状腺肿瘤病例（20 例滤泡性腺瘤、10 例滤泡状癌和 50 例乳头状癌），使用微卫星标记物和福尔马林固定石蜡包埋存档组织中的 DNA，检测染色体 3p14、9p21、13q21、6q22 和 17p13 等位基因缺失的 LOH。
滤泡性腺瘤、滤泡状癌和乳头状癌中分别有 10.5%、33.3%和 30.4%存在 3p14 的 LOH，6%、44.4%和 47.8%存在 9p21 的 LOH，5.3%、20.0%和 35.4%存在 pRb 基因的 LOH，5.3%、22.2%和 43.8%存在 E-钙黏蛋白基因的 LOH，0%、40%和 45.8%存在 17p13 的 LOH。FHIT 基因、p16、pRb、E-钙黏蛋白和 p53 基因的 LOH 在滤泡状癌和乳头状癌中比在滤泡性腺瘤中更常见。
五种抑癌基因（脆性组氨酸三联体基因、p16、pRb、E-钙黏蛋白和 p53）的 LOH 结果在良性肿瘤和恶性肿瘤之间存在统计学差异。在乳头状癌中，p16、E-钙黏蛋白和 p53 基因的 LOH 与低分化程度密切相关，E-钙黏蛋白的 LOH 与淋巴结转移有关。
