Strejan G H, Gilbert J J, St Louis J
Department of Microbiology and Immunology, University of Western Ontario, London, Canada.
Cell Immunol. 1988 Oct 1;116(1):250-6. doi: 10.1016/0008-8749(88)90225-0.
Guinea pig myelin basic protein (MBP)-liposomes were prepared and fixed with 0.2% glutaraldehyde (GA). Lewis rats were treated with glutaraldehyde-fixed MBP-liposomes (MBP-L-GA) or with cytochrome-c-liposomes (CYC-L-GA), 7 days before and 7 days after challenge with MBP in CFA. Rats treated with MBP-L-GA, but not with CYC-L-GA, were very well protected against the clinical manifestations of EAE. The protection was better than that obtained after treatment with conventional MBP-liposomes (without glutaraldehyde). Furthermore, when grown in vitro for 72 hr in the presence of MBP, lymphocytes from rats treated with MBP-L-GA and challenged with MBP in CFA exhibited a marked decrease in their ability to transfer EAE to normal syngeneic recipients.
制备豚鼠髓鞘碱性蛋白（MBP）脂质体，并用0.2%戊二醛（GA）固定。在弗氏完全佐剂（CFA）中用MBP攻击前7天和攻击后7天，给Lewis大鼠注射戊二醛固定的MBP脂质体（MBP-L-GA）或细胞色素c脂质体（CYC-L-GA）。用MBP-L-GA而非CYC-L-GA处理的大鼠对实验性自身免疫性脑脊髓炎（EAE）的临床表现具有很好的保护作用。这种保护作用优于用常规MBP脂质体（无戊二醛）处理后获得的保护作用。此外，在用MBP-L-GA处理并在CFA中用MBP攻击的大鼠的淋巴细胞，在MBP存在下体外培养72小时后，将EAE转移至正常同基因受体的能力显著下降。
