Sargon M F, Ogretmenoglu O, Gunenc Beser C, Karaoglan Y, Ercakmak B, Hayran H M, Hayran M, Kasirga U B
Department of Anatomy, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
Folia Morphol (Warsz). 2014 Feb;73(1):24-9. doi: 10.5603/FM.2014.0004.
The first aim of this study was the quantification of nerve fibres found in terminal branches of facial nerve and the second aim was the ultrastructural analysis of these terminal branches in order to observe their ultrastructural differences, if present. In the examination of literature; we could not find any studies related to this subject.
Four fresh frozen head and neck specimens were used and the dissections were done bilaterally. Therefore; totally 8 samples were examined. The samples were prepared according to routine transmission electron microscopic tissue preparation technique. The semi-thin sections were examined under light microscope by camera lucida. In every sample, the quantitative analysis was performed in 5 different areas in an area of 0.01 mm2 and statistical analysis was done. Secondly; the ultrastructural appearance of these terminal branches were examined under transmission electron microscope.
In the quantitative analysis of terminal branches of facial nerve in an area of 0.01 mm2; the least number of nerve fibres were found in temporal branches and the highest number were detected in cervical branches. In transmission electron microscopic examination, no significant difference was found in between these branches. In the statistical analysis; statistically significant differences were obtained in between the temporal and buccal, marginal mandibular, cervical branches; zygomatic and marginal mandibular, cervical branches; buccal and marginal mandibular, cervical branches; marginal mandibular and cervical branches.
These numerical data will have an importance during the nerve repair process of terminal branches of facial nerve in various injuries.
本研究的首要目的是对面神经终末支中的神经纤维进行定量分析，第二个目的是对这些终末支进行超微结构分析，以观察其超微结构差异（若存在）。在文献检索中，我们未找到任何与此主题相关的研究。
使用4个新鲜冷冻的头颈部标本，双侧进行解剖。因此，共检查了8个样本。样本按照常规透射电子显微镜组织制备技术进行制备。半薄切片在光学显微镜下通过摄像描绘器进行检查。在每个样本中，在0.01平方毫米的区域内的5个不同区域进行定量分析，并进行统计分析。其次，在透射电子显微镜下检查这些终末支的超微结构外观。
在0.01平方毫米区域内对面神经终末支进行定量分析时，颞支中发现的神经纤维数量最少，颈支中检测到的数量最多。在透射电子显微镜检查中，这些分支之间未发现显著差异。在统计分析中，颞支与颊支、下颌缘支、颈支之间；颧支与下颌缘支、颈支之间；颊支与下颌缘支、颈支之间；下颌缘支与颈支之间获得了统计学显著差异。
这些数值数据在面神经终末支在各种损伤中的神经修复过程中将具有重要意义。
