Jamshidian Hassan, Hashemi Mohsen, Nowroozi Mohammad Reza, Ayati Mohsen, Bonyadi Mahdieh, Najjaran Tousi Vahid
Department of Uro-oncology, Imam Khomeini Hospital, Tehran University of Medical Sciences,Tehran,
Department of Uro-oncology, Imam Khomeini Hospital, Tehran University of Medical Sciences,Tehran,Iran.
Urol J. 2014 Mar 3;11(1):1232-7.
To the assess sensitivity and specificity of urinary levels of hyaluronic acid (HA) and hyaluronidase (HAase) as an individual or a combined test to diagnose bladder transitional cell carcinoma (TCC).
One hundred and ninety-four urine specimens were collected from individuals between July 2007 and March 2008. The urinary level of hyaluronic acid (HA) was measured by Enzyme-linked immunosorbent assay. Thereafter, the urinary levels of HA and HAase were normalized to urinary creatinine level and expressed as ng/mg and μ/mg.
Eighty percent of patients with bladder cancer had urinary HA level < 500 ng/mg, and 90% of controls showed HA level < 500 ng/mg (P < .001). The mean urinary levels of HA in controls did not vary significantly (P < .05), whereas they significantly increased (2.5 to 6.5 folds) in all grades of TCC. More than 80% of patients with grades 2 and 3 TCC had urinary HAase level < 10 μ/mg and over 80% of controls showed HAase level < 10 μ/mg (P < .05). Hyaluronidase levels increased in patients with grades 2 and 3 bladder TCC.
Measurement of urinary levels of HA and HAase (with 89% sensitivity and 83% specificity) appears to be a highly accurate and non-invasive method for detecting bladder TCC and evaluating its grade.
评估尿中透明质酸（HA）和透明质酸酶（HAase）水平作为单独或联合检测手段诊断膀胱移行细胞癌（TCC）的敏感性和特异性。
2007年7月至2008年3月间收集了194份个体尿液标本。采用酶联免疫吸附测定法测量尿中透明质酸（HA）水平。此后，将尿中HA和HAase水平标准化为尿肌酐水平，并以ng/mg和μ/mg表示。
80%的膀胱癌患者尿HA水平<500 ng/mg，90%的对照组患者HA水平<500 ng/mg（P<.001）。对照组尿HA平均水平无显著差异（P<.05），而在所有分级的TCC中均显著升高（2.5至6.5倍）。80%以上2级和3级TCC患者尿HAase水平<10 μ/mg，80%以上对照组HAase水平<10 μ/mg（P<.05）。2级和3级膀胱TCC患者透明质酸酶水平升高。
测量尿中HA和HAase水平（敏感性89%，特异性83%）似乎是检测膀胱TCC及其分级的一种高度准确且无创的方法。
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