1st University Urology Clinic, Laiko Hospital, University of Athens, Ag.Thoma 17, 11527, Athens, Greece.
Department of Urology, Hadassah Hebrew University Medical Center, Jerusalem, Israel.
Med Oncol. 2018 May 25;35(7):97. doi: 10.1007/s12032-018-1157-9.
The goal of the study is to examine the possible use of HA (hyaluronic acid) and HAase (hyaluronidase) as novel urine biomarkers for the early diagnosis for prostate cancer (Pca). After a prostatic massage, the urine of 118 high-risk patients for Pca was collected, and the patients were submitted to ultrasound-guided transrectal biopsy. HA and HAase were detected and analyzed with Enzyme-Linked Immunosorbent Assay, and a statistical analysis of the urine levels of the two biomarkers according to the histology results was performed. HAase and HA were independently associated with Pca, and both HAase and HA showed significant predictive ability for prostate cancer. With an optimal cut-off point of 183.71 HAase had 70% sensitivity maintaining at the same time a 55.2% specificity, while the optimal cut-off point for HA was 50.13 with 65% sensitivity and 53.9% specificity. Patients with HAase more than 183.71 ng/ml had 3.67 times greater likelihood for prostate cancer and Patients with HA more than 50.13 ng/ml had 2.31 times greater likelihood for prostate cancer. The need of novel biomarkers that will improve the efficacy of PSA is urgent. HAase and HA showed significant predictive ability for prostate cancer and were independently associated with Pca, and greater levels were associated with greater odds for prostate cancer. To Our Knowledge, this is the first study referring to the detection of HAase and HA as potential urine biomarkers for the early diagnosis of Pca.
本研究旨在探讨透明质酸（HA）和透明质酸酶（HAase）作为前列腺癌（Pca）早期诊断新型尿生物标志物的可能性。对 118 例前列腺癌高危患者进行前列腺按摩后，采集尿液，行超声引导下经直肠前列腺穿刺活检。采用酶联免疫吸附试验（ELISA）检测和分析 HA 和 HAase，并根据组织学结果对两种生物标志物的尿液水平进行统计学分析。HAase 和 HA 与 Pca 独立相关，且两者均对前列腺癌具有显著的预测能力。HAase 的最佳截断值为 183.71 ng/ml 时，敏感性为 70%，特异性为 55.2%，而 HA 的最佳截断值为 50.13 ng/ml 时，敏感性为 65%，特异性为 53.9%。HAase 大于 183.71 ng/ml 的患者患前列腺癌的可能性增加 3.67 倍，HA 大于 50.13 ng/ml 的患者患前列腺癌的可能性增加 2.31 倍。迫切需要新型生物标志物来提高 PSA 的疗效。HAase 和 HA 对前列腺癌具有显著的预测能力，且与 Pca 独立相关，水平越高，患前列腺癌的几率越大。据我们所知，这是首次研究检测 HAase 和 HA 作为前列腺癌早期诊断的潜在尿生物标志物。
