Lei Bo, Shin Kwan-Ha, Koh Young-Hag, Kim Hyoun-Ee
Department of Dental Laboratory Science and Engineering, Korea University, Seoul, 136703, South Korea.
J Biomed Mater Res B Appl Biomater. 2014 Oct;102(7):1528-36. doi: 10.1002/jbm.b.33133. Epub 2014 Mar 5.
We produced highly porous gelatin-siloxane (GLA-S) hybrid scaffolds with biomimetic anisotropic porous structure, physiochemical properties, mechanical behaviors and biological functions by treating gelatin-siloxane hybrid gels in an ammonium hydroxide solution. The siloxane used as an inorganic phase could effectively crosslink the gelatin polymer, which allowed for the unidirectional enlargement of ammonia vacuoles during ammonium hydroxide treatment. This created aligned pores in an axial direction when the siloxane contents (10 and 20 wt %) were high. In addition, the gelatin polymer could be uniformly hybridized with the siloxane phase at the molecular level, while intense interaction between these two phases could be achieved. This resulted in a significant increase in mechanical properties. The GLA-S hybrid scaffold with a siloxane content of 10 wt % showed reasonably high compressive yield strength of 4.2 ± 0.1 MPa and compressive modulus of 84 ± 5 MPa at a porosity of 86 vol %, which would be comparable to those of natural cancellous bone. In addition, the GLA-S hybrid scaffold had good biocompatibility assessed by in vitro cell tests using pre-osteoblast MC3T3-E1 cells.
我们通过在氢氧化铵溶液中处理明胶 - 硅氧烷混合凝胶，制备出了具有仿生各向异性多孔结构、物理化学性质、力学行为和生物学功能的高度多孔明胶 - 硅氧烷（GLA - S）混合支架。用作无机相的硅氧烷能够有效地交联明胶聚合物，这使得在氢氧化铵处理过程中氨泡单向扩大。当硅氧烷含量（10重量％和20重量％）较高时，会在轴向形成排列整齐的孔隙。此外，明胶聚合物能够在分子水平上与硅氧烷相均匀杂交，同时这两个相之间能够实现强烈的相互作用。这导致力学性能显著提高。硅氧烷含量为10重量％的GLA - S混合支架在孔隙率为86体积％时，显示出相当高的抗压屈服强度，为4.2±0.1MPa，抗压模量为84±5MPa，这与天然松质骨相当。此外，通过使用前成骨细胞MC3T3 - E1细胞进行的体外细胞测试评估，GLA - S混合支架具有良好的生物相容性。
