Tsai W P, Oroszlan S
Laboratory of Molecular Virology and Carcinogenesis, NCI-Frederick Cancer Research Facility, Maryland 21701.
Virology. 1988 Oct;166(2):608-11. doi: 10.1016/0042-6822(88)90535-1.
The major mature env-gene products of avian reticuloendotheliosis-associated virus (REV-A) are the surface glycoprotein (gp90) and the transmembrane protein (gp20). We have previously reported that gp90 was detected in the REV-A virus by Western blot analysis as well as in the REV-A-infected cells by radioimmunoprecipitation with antibodies raised in rabbits against the gp90 C-terminal tridecapeptide which was predicted from the nucleotide sequence (Wilhemsen et al., J. Virol., 52, 172, 1984). We have now shown that this antibody detected antigens on the REV-A-infected cells by fluorescence-activated cell sorter (FACS) analysis, and conferred specific cytotoxic effects on the infected cells in the presence of rabbit complement using the chromium release assay. These results clearly indicate that the C-terminal epitope of gp90 is situated on the surface of the REV-A-infected cells and accessible to site-directed antibodies which cause cytotoxicity by activating the complement system. The possible in vivo roles of this antibody are discussed.
禽网状内皮组织增生症相关病毒（REV-A）主要的成熟env基因产物是表面糖蛋白（gp90）和跨膜蛋白（gp20）。我们之前报道过，通过蛋白质免疫印迹分析在REV-A病毒中检测到了gp90，并且用针对从核苷酸序列预测的gp90 C端十三肽在兔体内产生的抗体，通过放射免疫沉淀法在REV-A感染的细胞中也检测到了gp90（Wilhemsen等人，《病毒学杂志》，52卷，172页，1984年）。我们现在已经表明，该抗体通过荧光激活细胞分选仪（FACS）分析在REV-A感染的细胞上检测到了抗原，并且在兔补体存在的情况下，使用铬释放试验对感染细胞赋予了特异性细胞毒性作用。这些结果清楚地表明，gp90的C端表位位于REV-A感染细胞的表面，并且可被通过激活补体系统引起细胞毒性的定点抗体识别。本文讨论了该抗体在体内可能的作用。
