Xu Hong, Yang Yanfeng, Zhou Caiyun, Huang Xiufeng, Lin Jun, Zhang Xinmei
Women's Hospital, Zhejiang University School of Medicine, Hangzhou, People's Republic of China.
Histol Histopathol. 2014 Sep;29(9):1153-60. doi: 10.14670/HH-29.1153. Epub 2014 Mar 5.
Abnormal endometrial expression of CC-chemokine receptor-1 (CCR1) may play a role in the pathogenesis of endometriosis. Adenomyosis, also called endometriosis interna, occurs when the endometrium invades the myometrium. The objective of this study was to determine CCR1 expression in endometrium in women with adenomyosis as compared to women without adenomyosis. We evaluated endometrial mRNA and protein expression in women with and without adenomyosis using quantitative polymerase chain reaction (PCR), immunohistochemical staining and western blot analysis, respectively. We detected CCR1-immunoreactive expression in endometrium in all women with and without adenomyosis. CCR1-immunoreactive staining in endometrial cells was significantly higher in women with adenomyosis (4.89±1.06) compared to those without adenomyosis (2.21±1.16, P<0.001). Women with adenomyosis had higher levels of CCR1 mRNA in endometrium compared to women without adenomyosis (P<0.05). CCR1 protein levels in endometrium were significantly higher in women with adenomyosis (1.66±0.79) compared to women without adenomyosis (0.56±0.13, P<0.001), and positively correlated with the severity of dysmenorrhea (r=0.87, P<0.001). These results suggest that increased CC-chemokine receptor expression may play a role in the pathogenesis of adenomyosis.
CC趋化因子受体1（CCR1）在内膜中的异常表达可能在子宫内膜异位症的发病机制中起作用。子宫腺肌病，也称为内在性子宫内膜异位症，是指子宫内膜侵入子宫肌层时发生的疾病。本研究的目的是确定子宫腺肌病患者与非子宫腺肌病患者子宫内膜中CCR1的表达情况。我们分别使用定量聚合酶链反应（PCR）、免疫组织化学染色和蛋白质印迹分析评估了有或无子宫腺肌病女性的子宫内膜mRNA和蛋白质表达。我们在所有有或无子宫腺肌病的女性子宫内膜中均检测到CCR1免疫反应性表达。与无子宫腺肌病的女性（2.21±1.16，P<0.001）相比，子宫腺肌病女性子宫内膜细胞中的CCR1免疫反应性染色显著更高（4.89±1.06）。与无子宫腺肌病的女性相比，子宫腺肌病女性子宫内膜中的CCR1 mRNA水平更高（P<0.05）。与无子宫腺肌病的女性（0.56±0.13，P<0.001）相比，子宫腺肌病女性子宫内膜中的CCR1蛋白水平显著更高（1.66±0.79），并且与痛经严重程度呈正相关（r=0.87，P<0.001）。这些结果表明，CC趋化因子受体表达增加可能在子宫腺肌病的发病机制中起作用。
