Haloactinopolyspora alkaliphila sp. nov., and emended description of the genus Haloactinopolyspora.

A facultatively alkaliphilic actinomycete strain, designated EGI 80088(T), was isolated from a saline-alkali soil sample from Xinjiang province, north-west China, and subjected to a polyphasic taxonomic characterization. Strain EGI 80088(T) formed fragmented aerial hyphae and short spore chains, and rod-like spores aggregated at maturity. Whole-cell hydrolysates of the isolate contained ll-diaminopimelic acid as the diagnostic diamino acid, and glucosamine, mannose, galactose, glucose and rhamnose as the marker sugars. The major fatty acids identified (>5%) were anteiso-C(15 : 0), iso-C(15 : 0), summed feature 4 (iso-C(17 : 1)I/anteiso-C(17 : 1)B), iso-C(16 : 0) and anteiso-C(17 : 0). The predominant menaquinone was MK-9(H4). The G+C content of the genomic DNA of strain EGI 80088(T) was 70.6 mol%. EGI 80088(T) showed the highest 16S rRNA gene sequence similarity to its closest phylogenetic neighbour Haloactinopolyspora alba YIM 93246(T) (98.5%). The DNA-DNA relatedness value of the strain EGI 80088(T) and H. alba YIM 93246(T) was 59.3±5.2%. On the basis of morphological, chemotaxonomic and phylogenetic characteristics and DNA-DNA hybridization data, strain EGI 80088(T) represents a novel species of the genus Haloactinopolyspora, for which the name Haloactinopolyspora alkaliphila sp. nov. (type strain EGI 80088(T) = BCRC 16946(T) = JCM 19128(T)) is proposed. The description of the genus Haloactinopolyspora has also been emended.