一种基于香豆素的高选择性铝离子开启型荧光探针及其体内应用。
A highly selective turn-on fluorescent probe for Al(III) based on coumarin and its application in vivo.
作者信息
Xiao Hongde, Chen Kun, Jiang Nannan, Cui Dandan, Yin Gui, Wang Jie, Wang Ruiyong
机构信息
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, People's Republic of China.
出版信息
Analyst. 2014 Apr 21;139(8):1980-6. doi: 10.1039/c3an02247a.
In this study, a turn-on coumarin-based fluorescent probe, 7-hydroxy-6-[(2-hydroxy-naphthalen-1-ylmethylene)-amino]-4-methyl-chroman-2-one (CN), was developed for detecting Al(3+) in aqueous systems. The binding ratio of CN-Al(3+) complexes was determined from the Job plot and ESI-MS data to be 1 : 1. The binding constant (Ka) of Al(3+) binding to CN was calculated to be 9.55 × 10(4) M(-1) from a Benesi-Hildebrand plot and the detection limit was evaluated to be as low as 0.10 μM (LOD = 3σ/slope). CN could be used as an effective fluorescent probe for detecting Al(3+) in living HeLa cells. Moreover, CN could also be applied in the in vivo detection of Al(3+) in zebrafish.
在本研究中，开发了一种基于香豆素的开启型荧光探针7-羟基-6-[(2-羟基萘-1-基亚甲基)-氨基]-4-甲基-色满-2-酮（CN），用于检测水体系中的Al(3+)。通过Job曲线和电喷雾电离质谱（ESI-MS）数据确定CN-Al(3+)配合物的结合比为1:1。根据Benesi-Hildebrand曲线计算得出Al(3+)与CN的结合常数（Ka）为9.55×10(4) M(-1)，检测限评估低至0.10 μM（LOD = 3σ/斜率）。CN可作为一种有效的荧光探针用于检测活HeLa细胞中的Al(3+)。此外，CN还可应用于斑马鱼体内Al(3+)的检测。
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