Gallin M Y, Murray D, Lass J H, Grossniklaus H E, Greene B M
Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, OH 44106.
Arch Ophthalmol. 1988 Oct;106(10):1447-52. doi: 10.1001/archopht.1988.01060140611033.
To elucidate the pathogenic mechanisms involved in onchocercal sclerosing keratitis in humans, we developed a model of onchocercal interstitial keratitis in guinea pigs. Onchocerca volvulus antigens injected intrastromally into corneas of preimmunized Hartley guinea pigs induced an intense stromal keratitis with corneal edema, neovascularization, and infiltration with acute and chronic inflammatory cells. This reaction subsided after two weeks. Repeated intrastromal injection resulted in an exacerbation of the keratitis and ultimately in residual scarring. These findings are consistent clinically and histopathologically with the chronic interstitial keratitis observed in humans. To define which antigens induce the corneal reaction, O volvulus antigens were separated by molecular sieve chromatography and injected intrastromally. The highest activity was shown to reside in the fraction containing molecules of intermediate molecular weight. This model will be useful in defining O volvulus antigens and their role in sclerosing keratitis as well as in elucidating the immune mechanisms involved.
为了阐明人类盘尾丝虫性硬化性角膜炎的致病机制，我们建立了豚鼠盘尾丝虫性间质性角膜炎模型。将盘尾丝虫抗原基质内注射到预先免疫的哈特利豚鼠角膜中，可诱发强烈的基质性角膜炎，伴有角膜水肿、新生血管形成以及急性和慢性炎症细胞浸润。两周后这种反应消退。重复进行基质内注射会导致角膜炎加重，并最终形成残留瘢痕。这些发现与人类中观察到的慢性间质性角膜炎在临床和组织病理学上是一致的。为了确定哪些抗原可诱导角膜反应，通过分子筛色谱法分离盘尾丝虫抗原并进行基质内注射。结果显示，最高活性存在于含有中等分子量分子的组分中。该模型将有助于确定盘尾丝虫抗原及其在硬化性角膜炎中的作用，以及阐明其中涉及的免疫机制。
