Declau F, Moeneclaey L, Forton G, Marquet J
ENT Department, University of Antwerp, Wilrijk, Belgium.
Arch Otorhinolaryngol. 1988;245(4):218-20. doi: 10.1007/BF00463930.
A modified Luxol fast blue technique was used to study the development of the temporal bone. This staining method makes it possible to make a clear distinction between the primitive cartilage present and the new forming bone. Although these tissues both contain a significant amount of collagen, their staining properties with the Luxol dyes are widely dissimilar, due to the different physicochemical properties of the collagen types involved in these tissues. The differentiation of mesenchymatous tissue into ligaments and joints can also be very clearly demonstrated with this technique. In studying the endochondral ossification process of the otic capsule and middle ear, the modified Luxol fast blue stain is a valuable technique that is complementary to more conventional staining methods.
采用改良的卢氏固蓝技术研究颞骨的发育。这种染色方法能够清晰区分现存的原始软骨和新形成的骨组织。尽管这些组织都含有大量胶原蛋白，但由于这些组织中所含胶原蛋白类型的物理化学性质不同，它们对卢氏染料的染色特性差异很大。运用该技术还能非常清晰地展示间充质组织向韧带和关节的分化过程。在研究耳囊和中耳的软骨内成骨过程时，改良的卢氏固蓝染色是一项有价值的技术，可作为对更传统染色方法的补充。
