Hu C F, van Huystee R B
Department of Plant Sciences, University of Western Ontario, London, Canada.
Biochem Biophys Res Commun. 1988 Oct 14;156(1):500-5. doi: 10.1016/s0006-291x(88)80869-6.
The epitope sites on the cationic peanut peroxidase were characterized by four monoclonal antibodies raised against this isozyme. Evidence is presented showing that the epitope for monoclonal antibody 1B is located on the polypeptide. Sensitivity of the epitopes recognized by 1M and 2F to 0.1M HCl, boiling, 10 mM periodate and trifluoromethane sulfonic acid treatment indicate that they occur at regions where oligosaccharides are linked to the polypeptide backbone. The antigenic specificity of 2A is, in addition, dependent on the conformation of the epitope site which is destroyed after partial proteolysis of the peroxidase.
针对阳离子花生过氧化物酶的表位位点通过四种针对该同工酶产生的单克隆抗体进行了表征。有证据表明，单克隆抗体1B的表位位于多肽上。单克隆抗体1M和2F识别的表位对0.1M盐酸、煮沸、10mM高碘酸盐和三氟甲磺酸处理的敏感性表明，它们出现在寡糖与多肽主链相连的区域。此外，2A的抗原特异性取决于表位位点的构象，该构象在过氧化物酶部分蛋白酶解后被破坏。
