Gingras M C, Szpirer J, Turcotte B, Bélanger L, Szpirer C
Le Centre de Recherche en Cancérologie de l'Université Laval, L'Hôtel-Dieu de Québec, Canada.
Cancer Res. 1988 Nov 15;48(22):6371-4.
Fetal rat hepatocytes and mouse hepatoma cells actively expressing alpha 1-fetoprotein (AFP) and albumin genes were fused with the use of Sendai virus, and the expression of normal (rat) and tumor (mouse) AFP and albumin genes was analyzed in hybrid clones. The tumor AFP gene and both albumin genes were active in 103 hybrids. Expression of the normal fetal rat AFP gene, however, was maintained in only 3 hybrids, and it was frequently lost or decreased selectively upon subcloning. Furthermore, the normal AFP gene, when expressed, was more reactive than the tumor AFP gene to repression by a glucocorticosteroid hormone. These results suggest constitutive differences in the manner an oncofetal gene is activated and regulated in normal and neoplastic states. AFP gene expression in normal hepatocytes appears to be subordinated to a differentiation program degenerated and bypassed in hepatoma cells.
利用仙台病毒将活跃表达α1-甲胎蛋白（AFP）和白蛋白基因的胎鼠肝细胞与小鼠肝癌细胞融合，并在杂交克隆中分析正常（大鼠）和肿瘤（小鼠）AFP及白蛋白基因的表达情况。肿瘤AFP基因和两个白蛋白基因在103个杂交细胞中均有活性。然而，正常胎鼠AFP基因仅在3个杂交细胞中得以维持表达，并且在亚克隆时经常选择性地丢失或表达降低。此外，正常AFP基因一旦表达，相较于肿瘤AFP基因，对糖皮质激素的抑制作用反应更为敏感。这些结果表明，癌胚基因在正常和肿瘤状态下的激活和调控方式存在组成性差异。正常肝细胞中的AFP基因表达似乎从属于一个在肝癌细胞中退化且被绕过的分化程序。
