Fukuzawa M, Ochiai H
Department of Botany, Faculty of Science, Hokkaido University.
J Biochem. 1988 May;103(5):884-8. doi: 10.1093/oxfordjournals.jbchem.a122366.
The preparation and properties of monoclonal antibodies against carbohydrate-binding proteins (discoidin I and discoidin II) in the cellular slime mold, Dictyostelium discoideum are described. Monoclonal antibody (mAb) ndI,II-1 bound both discoidins I and II specifically. mAb nI-1 and mAb dI-1 bound only discoidin I but their binding specificities were different: nI-1 recognized the native form and dI-1 the denatured form. mAb dII-1 bound only denatured discoidin II. In preliminary work mAbs dII-1 and nI-1 were found to be useful for localizing discoidins I and II immunohistochemically.
本文描述了针对细胞黏菌盘基网柄菌中碳水化合物结合蛋白（盘状菌素I和盘状菌素II）的单克隆抗体的制备及其特性。单克隆抗体（mAb）ndI,II-1能特异性结合盘状菌素I和II。mAb nI-1和mAb dI-1仅结合盘状菌素I，但它们的结合特异性不同：nI-1识别天然形式，dI-1识别变性形式。mAb dII-1仅结合变性的盘状菌素II。在初步研究中，发现mAbs dII-1和nI-1可用于免疫组织化学定位盘状菌素I和II。
