一种可提高抗原特异性单克隆抗体产生频率的免疫方案。

An immunisation protocol which enhances the frequency of antigen-specific monoclonal antibody production.

作者信息

Shibier O, Hampton S M, Marks V

机构信息

Department of Biochemistry, University of Surrey, Guildford, U.K.

出版信息

J Immunol Methods. 1988 Nov 10;114(1-2):49-52. doi: 10.1016/0022-1759(88)90152-4.

DOI:10.1016/0022-1759(88)90152-4

PMID:2460563

Abstract

An immunisation protocol has been developed for small molecular weight antigens which results in a high percentage of specific hybridomas being produced after cell fusion. An enzyme-linked immunosorbent assay (ELISA) was developed for screening the desired antibodies in the culture supernatants. A conventional immunisation regimen was followed by doses of antigen in sterile water on each of the last 4 days before fusion. A range of antigen doses was used and the specific efficiency of fusion was increased by selection of the optimum amount. The antigen used as a model antigen in these experiments was biosynthetic human insulin.

摘要

已针对小分子抗原制定了免疫方案，该方案可使细胞融合后产生的特异性杂交瘤比例很高。开发了一种酶联免疫吸附测定法（ELISA），用于筛选培养上清液中的所需抗体。在融合前的最后4天，每天用无菌水给予抗原剂量，遵循传统免疫方案。使用了一系列抗原剂量，并通过选择最佳量提高了融合的特异性效率。在这些实验中用作模型抗原的是生物合成人胰岛素。