The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan.
The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan.
Carbohydr Polym. 2014 Apr 15;104:199-203. doi: 10.1016/j.carbpol.2014.01.014. Epub 2014 Jan 10.
Production of CMCase from newly isolated strain of Aspergillus versicolor was studied by optimizing different physico-chemical parameters. Cellulolytic potential of the isolate was confirmed by the simple agar plate method and then subjected to variation of a single parameter at a time in CMC containing medium. Maximum enzyme production was obtained at 30°C in 120 h when medium was supplemented with 0.5 gm% carboxymethyl cellulose, 0.2 gm% Tween 80, 0.075 gm% peptone, 0.050 gm% CaCl2, 1.5 gm% NaNO3 and 0.2 gm% KH2PO4 at pH 4.0. Current study holds great potential as the organism reported here could easily be used for the production of cellulase using renewable biomass.
研究了从新分离的土曲霉中生产 CMCase,优化了不同的物理化学参数。通过简单的琼脂平板法证实了分离物的纤维素酶潜力,然后在含有 CMC 的培养基中一次改变一个参数。当培养基中补充 0.5gm%羧甲基纤维素、0.2gm%吐温 80、0.075gm%蛋白胨、0.050gm%氯化钙、1.5gm%硝酸钠和 0.2gm%磷酸氢二钾,pH 值为 4.0 时,在 30°C 下培养 120 小时可获得最大酶产量。本研究具有很大的潜力,因为这里报道的生物体可以很容易地用于使用可再生生物质生产纤维素酶。