Wang Xiaomin, He Kongwang, Zhang Wenwen, Zhou Zhongtao, Mao Aihua, Yu Zhengyu
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biologicals Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing, Jiangsu Province 210014, PR China.
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biologicals Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing, Jiangsu Province 210014, PR China.
Infect Genet Evol. 2014 Jun;24:193-201. doi: 10.1016/j.meegid.2014.02.006. Epub 2014 Mar 6.
Porcine reproductive and respiratory syndrome virus (PRRSV) has been reported to have evolved at a high evolutionary rate and the extensive genetic variation. In this study, 44 PRRSV positive cases obtained from different provinces of China were sequenced and analyzed. Comparative analysis of partial isolates based on nsp2 sequences revealed that highly pathogenic PRRSV were the dominant viruses in China from 2008 to 2010 and some novel strains with an extra deletion of 19aa. Phylogenetic analysis based on the GP5 genes showed that the PRRSV isolates from 1996 to 2010 had a great variation and the North American genotype was further divided into six subgenotypes. No apparent relationship between the heterogeneity and the geographic origin of isolates was observed. The 44 isolates and 29 representative strains were divided into six subgenotypes. Further analysis of the GP5 protein suggested that these strains of subgenotypes I, II and III exhibited variations in the primary neutralizing epitope and almost all isolates of subgenotypes II and III had more N-linked glycosylation sites. In addition, some mutations which could mirror the viral evolution and adaptation were also observed in this study. All these results might be useful to study the genetic variation and genetic relatedness among PRRSV strains in China.
据报道，猪繁殖与呼吸综合征病毒（PRRSV）进化速度快，存在广泛的基因变异。本研究对从中国不同省份获得的44例PRRSV阳性病例进行了测序和分析。基于nsp2序列对部分分离株进行比较分析发现，高致病性PRRSV在2008年至2010年期间是中国的优势病毒，并且存在一些额外缺失19个氨基酸的新型毒株。基于GP5基因的系统发育分析表明，1996年至2010年的PRRSV分离株变异很大，北美基因型进一步分为六个亚基因型。未观察到分离株的异质性与其地理来源之间存在明显关系。44株分离株和29株代表性毒株被分为六个亚基因型。对GP5蛋白的进一步分析表明，这些I、II和III亚基因型毒株在主要中和表位上存在变异，并且几乎所有II和III亚基因型分离株都有更多的N-连接糖基化位点。此外，本研究还观察到一些能够反映病毒进化和适应性的突变。所有这些结果可能有助于研究中国PRRSV毒株之间的基因变异和遗传相关性。
