Navarro Enrique, Muñiz Selene, Korkaric Muris, Wagner Bettina, de Cáceres Miquel, Behra Renata
Pyrenean Institute of Ecology (CSIC), Av. Montañana 1005, 50059 Zaragoza, Spain.
Pyrenean Institute of Ecology (CSIC), Av. Montañana 1005, 50059 Zaragoza, Spain.
J Photochem Photobiol B. 2014 Mar 5;132:94-101. doi: 10.1016/j.jphotobiol.2014.02.005. Epub 2014 Feb 15.
Although the biological importance of ultraviolet light (UVR) attenuation has been recognised in marine and freshwater environments, it is not generally considered in in vitro ecotoxicological studies using algal cell suspensions. In this study, UVA and UVB extinction were determined for cultures of algae with varying cell densities, and the data were used to calculate the corresponding extinction coefficients for both UVA and UVB wavelength ranges. Integrating the Beer-Lambert equation to account for changes in the radiation intensity reaching each depth, from the surface until the bottom of the experimental vessel, we obtained the average UVA and UVB intensity to which the cultured algal cells were exposed. We found that UVR intensity measured at the surface of Chlamydomonas reinhardtii cultures lead to a overestimation of the UVR dose received by the algae by 2-40 times. The approach used in this study allowed for a more accurate estimation of UVA and UVB doses.
尽管紫外线辐射（UVR）衰减在海洋和淡水环境中的生物学重要性已得到认可，但在使用藻类细胞悬浮液的体外生态毒理学研究中，通常并未予以考虑。在本研究中，测定了不同细胞密度的藻类培养物的UVA和UVB消光值，并利用这些数据计算了UVA和UVB波长范围内相应的消光系数。通过对比尔-朗伯方程进行积分，以考虑从实验容器表面直至底部各深度处辐射强度的变化，我们得出了培养的藻类细胞所暴露的平均UVA和UVB强度。我们发现，在莱茵衣藻培养物表面测得的UVR强度导致对藻类所接受的UVR剂量高估了2至40倍。本研究中使用的方法能够更准确地估算UVA和UVB剂量。
