Chang Lingling, She Ruiping, Ma Longhuan, You Hua, Hu Fengjiao, Wang Tongtong, Ding Xiao, Guo Zhaojie, Soomro Majid Hussain
Laboratory of Animal Pathology and Public Health, Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agriculture University, Beijing, PR China.
Laboratory of Animal Pathology and Public Health, Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agriculture University, Beijing, PR China.
Reprod Toxicol. 2014 Jul;46:1-11. doi: 10.1016/j.reprotox.2014.02.008. Epub 2014 Mar 4.
Eight-week-old male Kunming mice were administered either melamine (MA, 30, 140, or 700 mg/kg/day), a melamine and cyanuric acid mixture (MC, each at 15, 70, or 350 mg/kg/day), or vehicle (control) for 3 consecutive days. Testicular toxicity was evaluated on days 1 and 5 after the final exposure. The testicular and epididymal weights and serum testosterone level were significantly decreased in the highest MC group (350 mg/kg/day). Histopathologically, both MA and MC caused obvious lesions in the testis and epididymis, with significant increases in sperm abnormalities. By TEM, the blood-testis barrier was damaged dose dependently. TUNEL staining showed that both MA and MC induced increases in germ cell apoptosis. The Sertoli cell vimentin was collapsed in the treated animals as detected by immunohistochemical staining and Western blotting. This study demonstrated that both MA and MC treatments could disrupt the blood-testis barrier and cause a clear testicular toxicity.
将8周龄雄性昆明小鼠连续3天给予三聚氰胺（MA，30、140或700毫克/千克/天）、三聚氰胺与氰尿酸混合物（MC，各为15、70或350毫克/千克/天）或赋形剂（对照）。在末次暴露后第1天和第5天评估睾丸毒性。在最高剂量的MC组（350毫克/千克/天）中，睾丸和附睾重量以及血清睾酮水平显著降低。组织病理学检查显示，MA和MC均导致睾丸和附睾出现明显病变，精子异常显著增加。通过透射电子显微镜观察，血睾屏障呈剂量依赖性受损。TUNEL染色显示，MA和MC均诱导生殖细胞凋亡增加。免疫组织化学染色和蛋白质免疫印迹法检测显示，在接受处理的动物中，支持细胞波形蛋白结构破坏。本研究表明，MA和MC处理均可破坏血睾屏障并导致明显的睾丸毒性。
