亚慢性氯化铝对雄性大鼠生殖细胞生成和睾丸酶活性的影响。
Effects of sub-chronic aluminum chloride on spermatogenesis and testicular enzymatic activity in male rats.
机构信息
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun 130112, China.
Laoshan Agricultural Bureau Institute of Animal Health Supervision, Yantai 264003, China.
出版信息
Life Sci. 2014 Apr 25;102(1):36-40. doi: 10.1016/j.lfs.2014.02.035. Epub 2014 Mar 6.
AIMS
The aim of this study was to determine the effects of sub-chronic aluminum chloride (AlCl3) on spermatogenesis and testicular enzymatic activity in male rats.
MAIN METHODS
Forty Wistar male rats were randomly divided into four groups: control group (CG, 0), low-dose group (LG, 64.18 mg/kg BW AlCl3), mid-dose group (MG, 128.36 mg/kg BW AlCl3) and high-dose group (HG, 256.72 mg/kg BW AlCl3). The rats were orally administered with AlCl3 for 120 days. At the end of the experiment, the contents of Al, Fe, Cu and Zn, the enzyme activities of testicular acid phosphatase (ACP), succinate dehydrogenase (SDH), lactate dehydrogenase (LDH), lactate dehydrogenase isoenzyme (LDH-x), the sperm count and the sperm malformation rate were examined.
KEY FINDINGS
The results showed that the Al and Cu contents, sperm count and the enzyme activities of testicular ACP, SDH, LDH and LDH-x decreased, while the Zn and Fe contents and sperm malformation rate increased in AlCl3-treated rats.
SIGNIFICANCE
It suggests that sub-chronic AlCl3 disorders the balance of trace element and decreases the spermatogenesis and the activities of testicular enzymes, indicating that AlCl3 has adverse effect on the testicular function in male rats.
目的
本研究旨在确定亚慢性氯化铝（AlCl3）对雄性大鼠精子发生和睾丸酶活性的影响。
方法
将 40 只 Wistar 雄性大鼠随机分为 4 组：对照组（CG，0）、低剂量组（LG，64.18mg/kg BW AlCl3）、中剂量组（MG，128.36mg/kg BW AlCl3）和高剂量组（HG，256.72mg/kg BW AlCl3）。大鼠经口给予 AlCl3 120 天。实验结束时，检测 Al、Fe、Cu 和 Zn 的含量，睾丸酸性磷酸酶（ACP）、琥珀酸脱氢酶（SDH）、乳酸脱氢酶（LDH）、乳酸脱氢酶同工酶（LDH-x）的酶活性、精子计数和精子畸形率。
结果
结果表明，AlCl3 处理组大鼠的 Al 和 Cu 含量、精子计数以及睾丸 ACP、SDH、LDH 和 LDH-x 的酶活性降低，而 Zn 和 Fe 含量以及精子畸形率升高。
结论
提示亚慢性 AlCl3 扰乱了微量元素的平衡，降低了精子发生和睾丸酶的活性，表明 AlCl3 对雄性大鼠的睾丸功能有不良影响。
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