亚慢性氯化铝暴露对大鼠卵巢的影响。

Effects of sub-chronic aluminum chloride exposure on rat ovaries.

机构信息

College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.

Veterinary and Animal Husbandry Department, Liaoning Agricultural College, Liaoning 115009, China.

出版信息

Life Sci. 2014 Mar 28;100(1):61-6. doi: 10.1016/j.lfs.2014.01.081. Epub 2014 Feb 13.

DOI:10.1016/j.lfs.2014.01.081

PMID:24530740

Abstract

AIMS

This experiment investigated the effects of sub-chronic aluminum chloride (AlCl3) exposure on rat ovaries.

MAIN METHODS

Eighty female Wistar (5weeks old) rats, weighed 110-120g, were randomly divided into four treatment groups: control group (CG), low-dose group (LG, 64mg/kg BW AlCl3), mid-dose group (MG, 128mg/kg BW AlCl3) and high-dose group (HG, 256mg/kg BW AlCl3). The AlCl3 was administered in drinking water for 120days. The ovarian ultrastructure was observed. The activities of acid phosphatase (ACP), alkaline phosphatase (ALP), succinate dehydrogenase (SDH), Na(+)-K(+)-ATPase, Mg(2+)-ATPase and Ca(2+)-ATPase, the contents of Fe, Cu and Zn, and the protein expression of follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) in the ovary were determined.

KEY FINDINGS

The results showed that the structure of the ovary was disrupted, the activities of ALP, ACP, SDH, Na(+)-K(+)-ATPase, Mg(2+)-ATPase and Ca(2+)-ATPase, the contents of Zn, Fe and the protein expression of FSHR and LHR were lowered, and the content of Cu was increased in AlCl3-treated rats than those in control.

SIGNIFICANCE

The results indicate that sub-chronic AlCl3 exposure caused the damage of the ovarian structure, the disturbed metabolism of Fe, Zn and Cu and the decreased activities of Na(+)-K(+)-ATPase, Mg(2+)-ATPase and Ca(2+)-ATPase in the ovary, which could result in suppressed energy supply in the ovary. A combination of suppression of energy supply and reduction of expression of FSHR and LHR could inhibit ovulation and corpus luteum development, leading to infertility in female rats.

摘要

目的

本实验研究了亚慢性氯化铝（AlCl3）暴露对大鼠卵巢的影响。

方法

将 80 只 5 周龄、体重 110-120g 的雌性 Wistar 大鼠随机分为 4 个处理组：对照组（CG）、低剂量组（LG，64mg/kg BW AlCl3）、中剂量组（MG，128mg/kg BW AlCl3）和高剂量组（HG，256mg/kg BW AlCl3）。AlCl3 通过饮用水给予 120 天。观察卵巢超微结构。测定卵巢酸性磷酸酶（ACP）、碱性磷酸酶（ALP）、琥珀酸脱氢酶（SDH）、Na(+)-K(+)-ATP 酶、Mg(2+)-ATP 酶和 Ca(2+)-ATP 酶活性、Fe、Cu 和 Zn 含量，以及卵泡刺激素受体（FSHR）和黄体生成素受体（LHR）的蛋白表达。

结果

结果表明，卵巢结构被破坏，ALP、ACP、SDH、Na(+)-K(+)-ATP 酶、Mg(2+)-ATP 酶和 Ca(2+)-ATP 酶活性、Zn、Fe 含量以及 FSHR 和 LHR 的蛋白表达降低，Cu 含量增加。

结论

结果表明，亚慢性 AlCl3 暴露导致卵巢结构损伤，Fe、Zn 和 Cu 代谢紊乱，卵巢 Na(+)-K(+)-ATP 酶、Mg(2+)-ATP 酶和 Ca(2+)-ATP 酶活性降低，可能导致卵巢能量供应不足。能量供应抑制与 FSHR 和 LHR 表达减少的结合可能抑制排卵和黄体发育，导致雌性大鼠不孕。