Department of Biotechnology, Institute of Agrochemistry and Food Technology, Consejo Superior de Investigaciones Científicas (IATA-CSIC) , Agustí Escardino 7, 46980 Paterna, València, Spain.
J Agric Food Chem. 2014 Apr 2;62(13):2816-21. doi: 10.1021/jf500287t. Epub 2014 Mar 19.
Kresoxim-methyl is nowadays widely used to combat a diversity of common diseases affecting high-value crops. In this article, we report the development and characterization of two novel immunoassays for the analysis of this pioneer strobilurin fungicide, and for the first time, a validation study with food samples was performed. A direct and an indirect competitive immunoassay based on a new anti-kresoxim-methyl monoclonal antibody were developed for sensitive and specific chemical analysis. Optimized assays showed limits of detection of 0.1 μg/L. Fruit and vegetable samples were extracted with acetonitrile by the QuEChERS procedure and analyzed by the developed immunoassays after a simple dilution in buffer, affording limits of quantification below US and European maximum residue limits. Immunochemical results of samples from kresoxim-methyl-sprayed strawberry fields demonstrated good statistical agreement with gas chromatography coupled to mass spectrometry as reference technique.
甲氧基甲基 kresoxim 目前广泛用于防治影响高价值作物的多种常见疾病。在本文中，我们报告了两种新型免疫分析方法的开发和特性，用于分析这种开创性的 strobilurin 杀真菌剂，并且首次对食品样品进行了验证研究。基于新的抗甲氧基甲基 kresoxim 单克隆抗体，建立了直接和间接竞争免疫分析方法，用于灵敏和特异性的化学分析。优化后的分析方法的检测限为 0.1μg/L。采用 QuEChERS 法，用乙腈从水果和蔬菜样品中提取，用缓冲液简单稀释后，用所建立的免疫分析方法进行分析，定量限低于美国和欧洲的最大残留限量。用气相色谱-质谱联用作为参考技术对来自甲氧基甲基 kresoxim 喷雾草莓田的样品进行免疫化学分析，结果表明具有良好的统计学一致性。
