Tablin F, Schumacher T, Pombo M, Marion C T, Huang K, Norris J W, Jandrey K E, Kittleson M D
Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California Davis, Davis, CA.
J Vet Intern Med. 2014 Mar-Apr;28(2):411-8. doi: 10.1111/jvim.12325. Epub 2014 Feb 24.
Cats with hypertrophic cardiomyopathy (HCM) are at risk for development of systemic thromboembolic disease. However, the relationship between platelet activation state and cardiovascular parameters associated with HCM is not well described.
To characterize platelet activation by flow cytometric evaluation of platelet P-selectin and semiquantitative Western blot analysis of soluble platelet-endothelial cell adhesion molecule-1 (sPECAM-1).
Eight normal healthy cats (controls) owned by staff and students of the School of Veterinary Medicine and 36 cats from the UC Davis Feline HCM Research Laboratory were studied.
Platelet-rich plasma (PRP) was used for all flow cytometry studies. Platelet surface CD41 and P-selectin expression were evaluated before and after ADP stimulation. sPECAM-1 expression was evaluated by Western blot analysis of platelet-poor plasma that had been stabilized with aprotinin. Standard echocardiographic studies were performed.
Resting platelets from cats with severe HCM had increased P-selectin expression compared to controls, and expressed higher surface density of P-selectin reflected by their increased mean fluorescence intensities (MFI). Stimulation with ADP also resulted in significantly increased P-selectin MFI of platelets from cats with severe HCM. Increased P-selectin expression and MFI correlated with the presence of a heart murmur and end-systolic cavity obliteration (ESCO). sPECAM-1 expression from cats with moderate and severe HCM was significantly increased above those of control cats.
P-selectin and sPECAM expression may be useful biomarkers indicating increased platelet activation in cats with HCM.
患有肥厚型心肌病（HCM）的猫有发生全身性血栓栓塞性疾病的风险。然而，血小板活化状态与HCM相关心血管参数之间的关系尚未得到充分描述。
通过流式细胞术评估血小板P-选择素以及可溶性血小板-内皮细胞黏附分子-1（sPECAM-1）的半定量蛋白质印迹分析来表征血小板活化情况。
研究了兽医学院教职工和学生拥有的8只正常健康猫（对照组）以及来自加州大学戴维斯分校猫科HCM研究实验室的36只猫。
所有流式细胞术研究均使用富血小板血浆（PRP）。在ADP刺激前后评估血小板表面CD41和P-选择素的表达。通过对用抑肽酶稳定的乏血小板血浆进行蛋白质印迹分析来评估sPECAM-1的表达。进行标准超声心动图研究。
与对照组相比，患有严重HCM的猫的静息血小板P-选择素表达增加，其平均荧光强度（MFI）增加反映出P-选择素的表面密度更高。用ADP刺激也导致患有严重HCM的猫的血小板P-选择素MFI显著增加。P-选择素表达和MFI的增加与心脏杂音和收缩末期腔室闭塞（ESCO）的存在相关。中度和重度HCM猫的sPECAM-1表达明显高于对照猫。
P-选择素和sPECAM表达可能是表明HCM猫血小板活化增加的有用生物标志物。
