Viazova E P, Azhigirova M A, Shubalova A L, Groznaia T G, Vashkevich M G
Biull Eksp Biol Med. 1988 Oct;106(10):446-8.
The structural heterogeneity of crosslinked pyridoxalated hemoglobin (polyHb-PLP) have been investigated by the methods of gel-filtration, isoelectric focusing and immunoelectrophoresis. It was established that the heterogeneity of poly-Hb-PLP is mainly depicted by polydispersion of molecular weight and less by the changes in the isoelectric properties. The qualitative difference has been shown between the immunochemical properties of the native hemoglobin and poly-Hb-PLP, which gives the suggestion of the formation of new antigenic determinants as a result of chemical modification and of more expressed antigenicity of modified hemoglobin compared with native protein. Poly-Hb-PLP has wide antigenic spectrum that corresponds to its structural heterogeneity. The data obtained suggest that the decrease in the antigenic activity of poly-Hb-PLP can be achieved by the lowering of the part of high molecular weight components.
采用凝胶过滤、等电聚焦和免疫电泳方法研究了交联吡哆醛化血红蛋白（聚血红蛋白-吡哆醛磷酸酯，polyHb-PLP）的结构异质性。结果表明，聚血红蛋白-吡哆醛磷酸酯的异质性主要表现为分子量的多分散性，等电性质变化较小。已显示天然血红蛋白和聚血红蛋白-吡哆醛磷酸酯的免疫化学性质存在质的差异，这表明化学修饰导致了新抗原决定簇的形成，且与天然蛋白质相比，修饰血红蛋白的抗原性更强。聚血红蛋白-吡哆醛磷酸酯具有广泛的抗原谱，与其结构异质性相对应。所得数据表明，通过降低高分子量组分的比例可以实现聚血红蛋白-吡哆醛磷酸酯抗原活性的降低。
