Scott A L, Diala C, Moraga D A, Ibrahim M S, Redding L, Tamashiro W K
Department of Immunology and Infectious Diseases, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, Maryland 21205.
Exp Parasitol. 1988 Dec;67(2):307-23. doi: 10.1016/0014-4894(88)90078-1.
The molecules associated with the surface of adult Dirofilaria immitis were identified and characterized employing IODO-GEN-mediated surface labeling methods. D. immitis female and male parasites were found to have a limited number of surface-associated proteins (17.5, 16, and 14.5 kDa) and glycoproteins (49 and 20 kDa) which were readily extracted from parasite homogenates in the absence of detergent. The major surface labeled proteins and glycoproteins were antigenic in rabbits, but appeared to elicit only a weak humoral response in dogs with patent dirofilariasis. In addition, a 10- to 6-kDa surface-associated glycolipid was identified which may form a coat on the outside of the parasite and play a role in immune evasion. In immunoprecipitation experiments, the glycolipid was not recognized by the antibodies from rabbits exposed to the glycolipid or by antibodies in the sera of patently infected animals. The glycolipid and the 14.5-kDa surface protein were selectively released by the adult parasite during in vitro culture.
采用碘代乙酰氨基葡萄糖介导的表面标记方法，对成年犬恶丝虫表面相关分子进行了鉴定和表征。发现犬恶丝虫的雌、雄寄生虫表面相关蛋白（17.5 kDa、16 kDa和14.5 kDa）和糖蛋白（49 kDa和20 kDa）数量有限，在无去污剂的情况下，这些蛋白和糖蛋白很容易从寄生虫匀浆中提取出来。主要的表面标记蛋白和糖蛋白在兔体内具有抗原性，但在患有显性恶丝虫病的犬中似乎仅引发微弱的体液反应。此外，还鉴定出一种10至6 kDa的表面相关糖脂，它可能在寄生虫外部形成一层包膜，并在免疫逃避中发挥作用。在免疫沉淀实验中，该糖脂未被接触过该糖脂的兔抗体或显性感染动物血清中的抗体识别。在体外培养过程中，成年寄生虫选择性释放了该糖脂和14.5 kDa的表面蛋白。
