Department of Endocrinology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Department of Neurology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Biochem Biophys Res Commun. 2014 Apr 18;446(4):843-9. doi: 10.1016/j.bbrc.2014.02.123. Epub 2014 Mar 5.
Sleep deprivation (SD) has become a worldwide public health concern due to the many negative health consequences associated with suboptimal sleep. SD has been linked to a catabolic hormone signature, heart disease, hypertension, diabetes, and an increase in morbidity and mortality. Herein, we investigated the effects and mechanism of SD on cardiac and metabolic health and evaluated the impact of exogenously supplied IGF-1 on these symptoms. In the present study, we show that 5 days of acute SD negatively impacted all of the various indicators of cardiac and metabolic health. All symptoms of SD were ameliorated by daily administration of IGF-1, however. IGF-1 administration also reduced the phosphorylation of Akt and expression of Bax, a promoter of apoptosis. Conversely, the expression of Bcl-2, an inhibitor of apoptosis, was elevated by IGF-1, and all IGF-1 effects were suppressed by the PI3K/Akt inhibitor LY294002, reaffirming the importance of the PI3K/Akt pathway in the maintenance of cardiac and metabolic health.
睡眠剥夺（SD）已成为全球公共卫生关注的问题，因为睡眠不足与许多负面健康后果有关。SD 与分解代谢激素特征、心脏病、高血压、糖尿病以及发病率和死亡率的增加有关。在此，我们研究了 SD 对心脏和代谢健康的影响和机制，并评估了外源性供应 IGF-1 对这些症状的影响。在本研究中，我们表明，急性 SD 持续 5 天会对心脏和代谢健康的所有各种指标产生负面影响。然而，每天给予 IGF-1 可改善所有 SD 症状。IGF-1 给药还降低了 Akt 的磷酸化和促进细胞凋亡的 Bax 的表达。相反，凋亡抑制剂 Bcl-2 的表达被 IGF-1 上调，并且 IGF-1 的所有作用都被 PI3K/Akt 抑制剂 LY294002 抑制，再次证实了 PI3K/Akt 通路在维持心脏和代谢健康中的重要性。
