Wilhelm Imola, Fazakas Csilla, Tamás Andrea, Tóth Gábor, Reglődi Dóra, Krizbai István A
Institute of Biophysics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
J Mol Neurosci. 2014 Nov;54(3):469-76. doi: 10.1007/s12031-014-0260-4. Epub 2014 Mar 11.
Cerebral microvascular endothelial cells-coming in contact with pericytes and astrocytes-constitute the structural basis of the blood-brain barrier (BBB). The continuous belt of interendothelial tight junctions (TJs) and the presence of specific transport systems, enzymes, and receptors in the brain endothelium regulate the molecular and cellular traffic into the central nervous system. Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide having several cellular protective effects. However, little is known about the effects of PACAP on the cerebral endothelium and BBB functions. Here, we show that PACAP has no significant pro-survival role in cerebral microvascular endothelial cells; however, it improves the barrier properties of the brain endothelium. PACAP induces an increase in the transendothelial electrical resistance, which is the most important marker of the tightness of the TJs. Moreover, PACAP has a protective role against glucose deprivation- and oxidative stress-induced junctional damage in microvascular brain endothelial cells.
脑微血管内皮细胞与周细胞和星形胶质细胞接触，构成了血脑屏障（BBB）的结构基础。内皮细胞间紧密连接（TJs）的连续带以及脑内皮中特定转运系统、酶和受体的存在，调节着进入中枢神经系统的分子和细胞运输。垂体腺苷酸环化酶激活多肽（PACAP）是一种具有多种细胞保护作用的神经肽。然而，关于PACAP对脑内皮和血脑屏障功能的影响知之甚少。在这里，我们表明PACAP在脑微血管内皮细胞中没有显著的促生存作用；然而，它改善了脑内皮的屏障特性。PACAP诱导跨内皮电阻增加，这是紧密连接紧密程度的最重要标志。此外，PACAP对微血管脑内皮细胞中葡萄糖剥夺和氧化应激诱导的连接损伤具有保护作用。
