Horbach G J, van der Boom H, van Bezooijen C F, Yap S H
TNO Institute for Experimental Gerontology, Rijswijk, The Netherlands.
Life Sci. 1988;43(21):1707-14. doi: 10.1016/0024-3205(88)90482-1.
The rate of albumin gene transcription and the level of albumin RNA sequences in liver nuclei of rats of various ages were determined to investigate the regulation of age-related changes in levels of cytoplasmic albumin mRNA in rat liver. No change was observed with age in both the rate of albumin gene transcription and the amount of nuclear albumin RNA sequences, which suggests that the increase in cytoplasmic albumin mRNA content in the rat liver with age is caused by a decreased turnover of this messenger. The length of poly(A)-tails of rat liver cytoplasmic RNA with age was also examined. Although no change with age was observed in the poly(A)-tail length sedimenting at the peak fraction, a shift towards shorter poly(A)-tails was found with age in the overall poly(A)-tail length distribution.
为了研究大鼠肝脏中细胞质白蛋白mRNA水平随年龄变化的调控机制，测定了不同年龄大鼠肝细胞核中白蛋白基因转录速率和白蛋白RNA序列水平。结果发现，白蛋白基因转录速率和核内白蛋白RNA序列数量均未随年龄发生变化，这表明大鼠肝脏中细胞质白蛋白mRNA含量随年龄增加是由于该信使RNA的周转减少所致。同时还检测了大鼠肝脏细胞质RNA的聚腺苷酸尾长度随年龄的变化。尽管在峰值组分处沉降的聚腺苷酸尾长度未随年龄发生变化，但在总体聚腺苷酸尾长度分布中发现，随年龄增长聚腺苷酸尾长度有向更短方向偏移的趋势。
