Reddy M Rajender, Reddy Putta Venkat, Kumar Yata Praveen, Srishailam A, Nambigari Navaneetha, Satyanarayana S
Department of Chemistry, Osmania University, Hyderabad, 500007, India.
J Fluoresc. 2014 May;24(3):803-17. doi: 10.1007/s10895-014-1355-6. Epub 2014 Mar 11.
The novel ligand (dmbip) 2-(4-N, N-dimethylbenzenamine)1H-imidazo[4, 5-f][1, 10]phenanthroline and its complexes Ru(phen)2dmbip (1), Ru(bpy)2dmbip (2), Co(phen)2dmbip (3) and Co(bpy)2dmbip (4) [where phen = 1, 10-phenanthroline, bpy = 2, 2'-bipyridine], have been synthesized and characterized by elemental analysis, IR, UV-Vis, (1)H NMR, (13)C NMR and Mass spectra. The DNA binding properties of the complexes were investigated by absorption, emission, quenching studies, light switch "on and off", salt dependent, sensor (cation and anion) studies, viscosity measurements, cyclic voltammetry, molecular modeling and docking studies. The four complexes were screened for Photo cleavage of pBR322 DNA, antimicrobial activity and cytotoxicity. The experimental results indicate that the four complexes can intercalate into DNA base pairs. The DNA-binding affinities of these complexes follow the order Ru(phen)2dmbip > Co(phen)2dmbip > Ru(bpy)2dmbip > Co(bpy)2dmbip.
新型配体（dmbip）2-（4-N，N-二甲基苯胺基）-1H-咪唑并[4,5-f][1,10]菲咯啉及其配合物Ru（phen）2dmbip（1）、Ru（bpy）2dmbip（2）、Co（phen）2dmbip（3）和Co（bpy）2dmbip（4）[其中phen = 1,10-菲咯啉，bpy = 2,2'-联吡啶]已通过元素分析、红外光谱、紫外-可见光谱、（1）H核磁共振谱、（13）C核磁共振谱和质谱进行了合成与表征。通过吸收、发射、猝灭研究、光开关“开与关”、盐依赖性、传感器（阳离子和阴离子）研究、粘度测量、循环伏安法、分子建模和对接研究对配合物的DNA结合性质进行了研究。对这四种配合物进行了pBR322 DNA光裂解、抗菌活性和细胞毒性的筛选。实验结果表明，这四种配合物可插入DNA碱基对中。这些配合物与DNA的结合亲和力顺序为Ru（phen）2dmbip > Co（phen）2dmbip > Ru（bpy）2dmbip > Co（bpy）2dmbip。
