Wang Guowei, Zhao Fang, Gao Ying
Key Laboratory for Chemical Materials of Xinjiang Uygur Autonomous Region, Engineering Center for Chemical Materials of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Xinjiang, 832003, People's Republic of China.
Luminescence. 2014 Dec;29(8):1008-13. doi: 10.1002/bio.2651. Epub 2014 Feb 24.
A novel post-chemiluminescence (PCL) reaction was discovered when lercanidipine was injected into the CL reaction mixture of N-chlorosuccinimide with alkaline eosin Y in the presence of cetyltrimethylammonium bromide (CTAB), where eosin Y was used as the CL reagent and CTAB as the surfactant. Based on this observation, a simple and highly sensitive PCL method combined with a flow injection (FI) technique was developed for the assay of lercanidipine. Under optimum conditions, the CL signal was linearly related to the concentration of lercanidipine in the range 7.0 × 10(-10) to 3.0 × 10(-6) g/mL with a detection limit of 2.3 × 10(-10) g/mL (3σ). The relative standard deviation (RSD) was 2.1% for 1.0 × 10(-8) g/mL lercanidipine (n = 13). The proposed method had been applied to the estimation of lercanidipine in tablets and human serum samples with satisfactory results. The possible CL mechanism is also discussed briefly.
当在十六烷基三甲基溴化铵（CTAB）存在的情况下，将乐卡地平注入N - 氯代琥珀酰亚胺与碱性曙红Y的化学发光（CL）反应混合物中时，发现了一种新型的后化学发光（PCL）反应，其中曙红Y用作CL试剂，CTAB用作表面活性剂。基于此观察结果，开发了一种结合流动注射（FI）技术的简单且高灵敏度的PCL方法用于乐卡地平的测定。在最佳条件下，CL信号与乐卡地平浓度在7.0×10⁻¹⁰至3.0×10⁻⁶g/mL范围内呈线性关系，检测限为2.3×10⁻¹⁰g/mL（3σ）。对于1.0×10⁻⁸g/mL的乐卡地平，相对标准偏差（RSD）为2.1%（n = 13）。所提出的方法已应用于片剂和人血清样品中乐卡地平的测定，结果令人满意。还简要讨论了可能的CL机制。
