Institute of Reproductive Medicine and Population, Medical Research Center, Seoul, Korea Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea.
Choate Rosemary Hall, New Haven, CT, USA.
Reprod Sci. 2014 Oct;21(10):1312-8. doi: 10.1177/1933719114525269. Epub 2014 Mar 10.
Various chemicals have been reported to induce the differentiation of human embryonic stem cells (hESCs) into cardiomyocytes (CMs), however, their contributions to the functionality of hESC-derived CMs are still limited. In this study, we evaluated the effects of red ginseng extract (RGE), ginsenoside-Rb1 (gRb1, panaxadiol), and ginsenoside-Re (gRe, panaxatriol) on the differentiation of hESCs and the functionality of derived CMs. Undifferentiated hESCs were treated with 0.25 mg/mL RGE, 10 μmol/L gRb1, or 10 μmol/L gRe for 48 hours at the differentiation induction (early stage) or maturation (late stage) period. The expression of mesodermal and cardiac transcription factor genes was upregulated in the ginsenoside-treated groups from early stage. The expression of cardiac sarcomeric genes was significantly upregulated at the late stage. The gRb1- and gRe-treated groups upregulated the expression of potassium voltage-gated channel subfamily E member 1 (KCNE1) and the gRe-treated group showed a longer beating duration compared to the control. Taken together, ginsenosides may enhance the functionality of hESC-derived CMs in vitro.
各种化学物质已被报道可诱导人胚胎干细胞（hESCs）分化为心肌细胞（CMs），然而，它们对 hESC 来源的 CMs 功能的贡献仍然有限。在本研究中，我们评估了红参提取物（RGE）、人参皂苷-Rb1（gRb1，人参二醇）和人参皂苷-Re（gRe，人参三醇）对 hESC 分化和衍生的 CMs 功能的影响。未分化的 hESCs 在分化诱导（早期）或成熟（晚期）阶段用 0.25 mg/mL RGE、10 μmol/L gRb1 或 10 μmol/L gRe 处理 48 小时。在 Ginsenoside 处理组中，从早期开始，中胚层和心脏转录因子基因的表达上调。在晚期，心肌肌节基因的表达显著上调。与对照组相比，gRb1 和 gRe 处理组上调了钾电压门控通道亚家族 E 成员 1（KCNE1）的表达，而 gRe 处理组的搏动持续时间更长。综上所述，人参皂苷可能增强 hESC 来源的 CMs 的体外功能。
