两种针对人睾丸细胞产生的单克隆抗体的特性鉴定。
Characterization of two monoclonal antibodies raised against human testicular cells.
作者信息
Kurpisz M, Mapp P, Lukaszyk A, Ogilvie J, Festenstein H, Sachs J
机构信息
Polish Academy of Scineces, Department of Histology and Experimental Embryology, Medical School, Poznan.
出版信息
Andrologia. 1988 Jul-Aug;20(4):304-10.
Human testicular cells isolated from biopsy tissue were used for generation of monoclonal antibodies. Two hybridoma supernatants C3 and D4 were selected according to their reaction with sperm-precursor cells (immature sperms) in an enzyme-linked immunosorbent assay (ELISA). C3 reacted with testicular but no other tissue. D4 did not reveal any pattern of testicular staining in spite of its similarity to C3 in binding to sperm-precursor cells in ELISA and microcytotoxicity test.
从活检组织中分离出的人类睾丸细胞被用于单克隆抗体的制备。根据两种杂交瘤细胞上清液C3和D4在酶联免疫吸附测定（ELISA）中与精子前体细胞（未成熟精子）的反应，对其进行了筛选。C3与睾丸组织发生反应，但不与其他组织反应。尽管D4在ELISA和微量细胞毒性试验中与精子前体细胞的结合情况与C3相似，但在睾丸染色中未显示出任何染色模式。
Zotero 插件