Merheb Maxime, Vaiedelich Stéphane, Maniguet Thiérry, Hänni Catherine
a Biotechnology Department , American University of Ras Al Khaimah , Ras Al Khaimah , United Arab Emirates .
b Music Museum, Cité de la Musique , Paris , France , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):355-9. doi: 10.3109/19401736.2014.895988. Epub 2014 Mar 11.
Great ancient composers have endured many obstacles and constraints which are very difficult to understand unless we perform the restoration process of ancient music. Species identification in leather used during manufacturing is the key step to start such a restoration process in order to produce a facsimile of a museum piano. Our study reveals the species identification in the leather covering the hammer head in a piano created by Erard in 1802. This is the last existing piano similar to the piano that Beethoven used with its leather preserved in its original state. The leather sample was not present in a homogeneous piece, yet combined with glue. Using a DNA extraction method that avoids PCR inhibitors; we discovered that sheep and cattle are the origin of the combination. To identify the species in the leather, we focused on the amounts of mitochondrial DNA in both leather and glue and results have led us to the conclusion that the leather used to cover the hammer head in this piano was made of cattle hide.
伟大的古代作曲家曾历经诸多障碍与限制，除非我们进行古代音乐的复原工作，否则很难理解这些。在制造过程中用于皮革的物种鉴定是启动此类复原工作的关键步骤，以便制作出博物馆钢琴的复制品。我们的研究揭示了1802年埃拉尔制造的一架钢琴琴槌头上皮革的物种鉴定情况。这是现存最后一架与贝多芬使用过的钢琴类似且皮革保持原始状态的钢琴。皮革样本并非整块均匀的，而是与胶水混合在一起。使用一种避免PCR抑制剂的DNA提取方法，我们发现绵羊和牛是这种组合的来源。为了鉴定皮革中的物种，我们专注于皮革和胶水中线粒体DNA的含量，结果使我们得出结论，这架钢琴琴槌头覆盖的皮革是牛皮制成的。
