Varga Renáta, Janovszky Ágnes, Szabó Andrea, Garab Dénes, Bodnár Dóra, Boros Mihály, Neunzehn Jörg, Wiesmann Hans-Peter, Piffkó József
Department of Oral and Maxillofacial Surgery, University of Szeged, Szeged, Hungary.
Microcirculation. 2014 Aug;21(6):524-31. doi: 10.1111/micc.12128.
The periosteum plays an important role in bone physiology, but observation of its microcirculation is greatly limited by methodological constraints at certain anatomical locations. This study was conducted to develop a microsurgical procedure which provides access to the mandibular periosteum in rats.
Comparisons of the microcirculatory characteristics with those of the tibial periosteum were performed to confirm the functional integrity of the microvasculature. The mandibular periosteum was reached between the facial muscles and the anterior surface of the superficial masseter muscle at the external surface of the mandibular corpus; the tibial periosteum was prepared by dissecting the covering muscles at the anteromedial surface. Intravital fluorescence microscopy was used to assess the leukocyte-endothelial interactions and the RBCV in the tibial and mandibular periosteum. Both structures were also visualized through OPS and fluorescence CLSM.
The microcirculatory variables in the mandibular periosteum proved similar to those in the tibia, indicating that no microcirculatory failure resulted from the exposure technique.
This novel surgical approach provides simple access to the mandibular periosteum of the rat, offering an excellent opportunity for investigations of microcirculatory manifestations of dentoalveolar and maxillofacial diseases.
骨膜在骨生理学中发挥着重要作用，但在某些解剖位置，其微循环的观察受到方法学限制。本研究旨在开发一种显微手术方法，以暴露大鼠下颌骨骨膜。
将下颌骨骨膜的微循环特征与胫骨骨膜的进行比较，以确认微血管系统的功能完整性。在下颌骨体的外表面，通过面肌与咬肌浅层前表面之间到达下颌骨骨膜；通过解剖胫骨前内侧表面的覆盖肌肉来制备胫骨骨膜。采用活体荧光显微镜评估胫骨和下颌骨骨膜中的白细胞-内皮细胞相互作用及红细胞流速。还通过正交偏振光谱成像和荧光共聚焦激光扫描显微镜对这两种结构进行可视化观察。
下颌骨骨膜的微循环变量与胫骨的相似，表明暴露技术未导致微循环衰竭。
这种新颖的手术方法为暴露大鼠下颌骨骨膜提供了简便途径，为研究牙槽和颌面疾病的微循环表现提供了绝佳机会。
