成纤维细胞生长因子可增强绒癌JAR细胞中人绒毛膜促性腺激素的合成，且不依赖于有丝分裂刺激。

Fibroblast growth factor enhances human chorionic gonadotropin synthesis independent of mitogenic stimulation in Jar choriocarcinoma cells.

作者信息

Oberbauer A M, Linkhart T A, Mohan S, Longo L D

机构信息

Department of Physiology, Loma Linda University, California 92350.

出版信息

Endocrinology. 1988 Dec;123(6):2696-700. doi: 10.1210/endo-123-6-2696.

DOI:10.1210/endo-123-6-2696

PMID:2461850

Abstract

The Jar choriocarcinoma cell line was used as an in vitro placental cell model to determine the effects of polypeptide growth factors on hCG beta secretion. Epidermal and fibroblast growth factor (FGF) treatment of serum-free cultures stimulated hCG beta secretion 2.5- and 4.0-fold over basal serum-free control levels within a 15-h incubation period. Insulin-like growth factor I, nerve growth factor, and transforming growth factor-beta had no significant effect on hCG beta secretion. FGF at concentrations as low as 0.125 ng/ml significantly elevated medium hCG beta levels without increasing cell number or total cellular protein. FGF stimulation of secretion was not detectable until 2 h of treatment. Intracellular hCG beta remained constant (23%) relative to total hCG beta (cell plus medium) as total hCG beta increased 3-fold, suggesting that FGF stimulated de novo hCG beta synthesis. Insulin significantly augmented the FGF-induced hCG beta stimulation without stimulating hCG beta production itself.

摘要

将Jar绒毛膜癌细胞系用作体外胎盘细胞模型，以确定多肽生长因子对hCGβ分泌的影响。在无血清培养物中，用表皮生长因子和成纤维细胞生长因子（FGF）处理，在15小时的孵育期内，刺激hCGβ分泌比无血清基础对照水平高2.5倍和4.0倍。胰岛素样生长因子I、神经生长因子和转化生长因子-β对hCGβ分泌无显著影响。浓度低至0.125 ng/ml的FGF能显著提高培养基中hCGβ水平，而不增加细胞数量或总细胞蛋白。直到处理2小时后才能检测到FGF对分泌的刺激作用。随着总hCGβ增加3倍，相对于总hCGβ（细胞加培养基），细胞内hCGβ保持恒定（23%），这表明FGF刺激了hCGβ的从头合成。胰岛素显著增强了FGF诱导的hCGβ刺激作用，而其本身不刺激hCGβ的产生。