Chernobrovkin Alexey L, Zubarev Roman A
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden; SciLifeLab, Stockholm, Sweden.
PLoS One. 2014 Mar 11;9(3):e91433. doi: 10.1371/journal.pone.0091433. eCollection 2014.
Cell cultures used routinely in proteomic experiments may contain proteins from other species because of infection, transfection or just contamination. Since infection or contamination may affect the results of a biological experiment, it is important to test the samples for the presence of "alien" proteins. Usually cells are tested only for the most common infections, and most of the existing tests are targeting specific contaminations. Here we describe a three-step procedure for reliable untargeted detection of viral proteins using proteomics data, and recommend this or similar procedure to be applied to every proteomics dataset submitted for publication.
由于感染、转染或仅仅是污染，蛋白质组学实验中常规使用的细胞培养物可能含有来自其他物种的蛋白质。由于感染或污染可能会影响生物学实验的结果，因此检测样品中是否存在“外来”蛋白质非常重要。通常，细胞仅针对最常见的感染进行检测，并且现有的大多数检测都是针对特定的污染物。在这里，我们描述了一种使用蛋白质组学数据可靠地非靶向检测病毒蛋白的三步程序，并建议将此程序或类似程序应用于提交发表的每个蛋白质组学数据集。
