Pitcock J A, Lyons H, Brown P S, Rightsel W A, Muirhead E E
Department of Pathology, University of Tennessee, Memphis 38146.
Exp Mol Pathol. 1988 Dec;49(3):373-87. doi: 10.1016/0014-4800(88)90009-3.
The rat renal papillary interstitum which contains abundant proteoglycans is a unique area important in renal function. These proteoglycans were studied ultrastructurally by ruthenium red fixation and staining and phosphate-buffered fixation before and after enzyme digestion. A tissue culture of rat renomedullary interstitial cells, the predominant cell of the renal papillary interstitum, was studied for its ability to synthesize proteoglycans and the proteoglycans were then analyzed. Tissue slices of whole rat renal inner medulla were also evaluated for their synthetic ability. In combination, these studies indicate that the dominant glycosaminoglycan is hyaluronic acid. The tissue culture of rat renal medullary interstitial cells synthesized glycosaminoglycans and on analysis, hyaluronic acid was found to be the chief glycosaminoglycan secreted by the renomedullary interstitial cells. Combined with the removal of the proteoglycans from tissue by leech hyaluronidase and testicular hyaluronidase, this suggests that the dominant glycosaminoglycan is hyaluronic acid. Hyaluronic acid is also synthesized by the intact papilla confirming the findings with the tissue culture. However, in addition, sulfated glycosaminoglycans were also synthesized by the intact papilla, presumably the product of the noninterstitial components of the papilla.
大鼠肾乳头间质含有丰富的蛋白聚糖，是肾脏功能中一个重要的独特区域。通过钌红固定和染色以及酶消化前后的磷酸盐缓冲固定，对这些蛋白聚糖进行了超微结构研究。对大鼠肾髓质间质细胞（肾乳头间质的主要细胞）的组织培养物进行了研究，以考察其合成蛋白聚糖的能力，然后对蛋白聚糖进行了分析。还评估了整个大鼠肾内髓的组织切片的合成能力。综合这些研究表明，主要的糖胺聚糖是透明质酸。大鼠肾髓质间质细胞的组织培养物合成了糖胺聚糖，经分析发现，透明质酸是肾髓质间质细胞分泌的主要糖胺聚糖。结合用蚂蟥透明质酸酶和睾丸透明质酸酶从组织中去除蛋白聚糖的结果，这表明主要的糖胺聚糖是透明质酸。完整的乳头也能合成透明质酸，这证实了组织培养的结果。然而，此外，完整的乳头还能合成硫酸化糖胺聚糖，推测这是乳头非间质成分的产物。
