Padilla Carlos, Padilla Andrés, Lobos Olga
Universidad de Talca, Talca, Chile.
J Infect Dev Ctries. 2014 Mar 13;8(3):265-70. doi: 10.3855/jidc.3464.
Vaginitis is one of the most common reasons women visit a gynecologist. Escherichia coli has been isolated from women with vaginitis, but its role as a vaginal infection aetiological agent is controversial. This study aimed to detect virulence genes and determine the antimicrobial susceptibility of E. coli strains isolated from monomicrobial and polymicrobial cultures collected from women with vaginitis.
The presence of the following virulence genes: papC, hly, iucC, afa, fimH, neuC, sfa/foc, cnf1, usp, and ibeA in two E. coli groups was determined by PCR. The antibacterial susceptibility of strains was tested.
A higher percentage (93.3%) of isolated strains from monomicrobial cultures with virulence genes in relation to polymicrobial cultures (56.7%) was found. The most frequent virulence genes in both groups were hly (p = 0.0357), fimH (p = 0.000), and cfn1 (p = 0.000). In addition, E. coli isolated from monomicrobial cultures showed 5 genetic combinations compared to the 10 observed in the polymicrobial cultures. An increased number of strains were sensitive to cefotaxime, moxifloxacin, and ciprofloxacin. A high resistance to trimethoprim-sulfamethoxazole was observed.
Most of the E. coli strains isolated from monomicrobial cultures and some from polymicrobial cultures showed virulence genes. A better understanding of the virulence and antibacterial susceptibility of E. coli strains isolated from patients with vaginitis can contribute to improved diagnosis and treatment of this disease.
阴道炎是女性看妇科医生最常见的原因之一。从阴道炎女性患者中分离出了大肠杆菌，但其作为阴道感染病原体的作用存在争议。本研究旨在检测从阴道炎女性患者的单一微生物和多微生物培养物中分离出的大肠杆菌菌株的毒力基因，并确定其抗菌药敏性。
通过聚合酶链反应（PCR）测定两组大肠杆菌中以下毒力基因的存在情况：papC、hly、iucC、afa、fimH、neuC、sfa/foc、cnf1、usp和ibeA。对菌株进行了抗菌药敏试验。
发现单一微生物培养物中分离出的带有毒力基因的菌株百分比（93.3%）高于多微生物培养物（56.7%）。两组中最常见的毒力基因是hly（p = 0.0357）、fimH（p = 0.000）和cfn1（p = 0.000）。此外，从单一微生物培养物中分离出的大肠杆菌显示出5种基因组合，而多微生物培养物中观察到10种。对头孢噻肟、莫西沙星和环丙沙星敏感的菌株数量增加。观察到对甲氧苄啶-磺胺甲恶唑有高耐药性。
从单一微生物培养物中分离出的大多数大肠杆菌菌株以及一些从多微生物培养物中分离出的菌株显示有毒力基因。更好地了解从阴道炎患者中分离出的大肠杆菌菌株的毒力和抗菌药敏性有助于改善该疾病的诊断和治疗。
