Sagirli Olcay, Toker Sıdıka Erturk, Önal Armağan
Department of Analytical Chemistry, Faculty of Pharmacy, Istanbul University, 34116, Beyazit, Istanbul, Turkey.
Luminescence. 2014 Dec;29(8):1014-8. doi: 10.1002/bio.2652. Epub 2014 Mar 12.
A new, sensitive and selective spectrofluorimetric method has been developed for the determination of duloxetine (DLX) in capsule and spiked human plasma. DLX, as a secondary amine compound, reacts with 7-chloro-4-nitrobenzofurazon (NBD-Cl), a highly sensitive fluorogenic and chromogenic reagent used in many investigations. The method is based on the reaction between the drug and NBD-Cl in borate buffer at pH 8.5 to yield a highly fluorescent derivative that is measured at 523 nm after excitation at 478 nm. The fluorescence intensity was directly proportional to the concentration over the range 50-250 ng/mL. The reaction product was also measured spectrophotometrically. The relation between the absorbance at 478 nm and the concentration is rectilinear over the range 1.0-12.0 µg/mL. The methods were successfully applied for the determination of this drug in pharmaceutical dosage form. The spectrofluorimetric method was also successfully applied to the determination of duloxetine in spiked human plasma. The suggested procedures could be used for the determination of DLX in pure form, capsules and human plasma being sensitive, simple and selective.
已开发出一种新型、灵敏且具选择性的荧光分光光度法，用于测定胶囊及加标人血浆中的度洛西汀（DLX）。DLX作为仲胺化合物，可与7-氯-4-硝基苯并呋咱（NBD-Cl）发生反应，NBD-Cl是一种在诸多研究中使用的高灵敏度荧光和显色试剂。该方法基于药物与NBD-Cl在pH 8.5的硼酸盐缓冲液中发生反应，生成一种高荧光衍生物，在478 nm激发后于523 nm处进行测定。荧光强度在50 - 250 ng/mL范围内与浓度成正比。反应产物也采用分光光度法进行测定。在1.0 - 12.0 µg/mL范围内，478 nm处的吸光度与浓度呈线性关系。这些方法已成功应用于药物剂型中该药物的测定。荧光分光光度法也成功应用于加标人血浆中度洛西汀的测定。所建议的方法可用于测定纯形式的DLX，用于胶囊和人血浆时灵敏、简便且具选择性。
