John Liza B, Chee Tess M, Gilham David E, Darcy Phillip K
Cancer Immunology Program, Peter MacCallum Cancer Centre, East Melbourne, VIC, Australia.
Methods Mol Biol. 2014;1139:177-87. doi: 10.1007/978-1-4939-0345-0_16.
Genetic modification of primary mouse T cells with chimeric antigen receptors (CAR) has emerged as an important tool for optimizing adoptive T cell immunotherapy strategies. However, limitations in current protocols for generating highly pure and sufficient numbers of enriched effector and helper CAR(+) T cell subsets remain problematic. Here, we describe a new retroviral transduction protocol for successfully generating transduced CD8(+) and CD4(+) T lymphocytes for in vitro and in vivo characterization.
用嵌合抗原受体（CAR）对原代小鼠T细胞进行基因改造已成为优化过继性T细胞免疫治疗策略的重要工具。然而，目前用于产生高纯度和足够数量的富集效应性和辅助性CAR(+) T细胞亚群的方案存在局限性，仍然是个问题。在此，我们描述了一种新的逆转录病毒转导方案，用于成功产生经转导的CD8(+)和CD4(+) T淋巴细胞，以进行体外和体内特性分析。
