Joshi Vijaya Bharti, Geary Sean M, Salem Aliasger K
College of Pharmacy, University of Iowa, Iowa City, IA, USA.
Methods Mol Biol. 2014;1139:453-66. doi: 10.1007/978-1-4939-0345-0_35.
Particle-based cancer vaccines prepared from biodegradable polymers are a potentially attractive way of delivering antigen alone, or in combination with adjuvant molecules, to dendritic cells (DC). Non-viral modes of vaccination must ensure the activation of cellular immune responses in addition to humoral responses if an effective antitumor immune response is to be initiated. Here we describe in detail a method for manufacturing poly(D,L-lactide-co-glycolide) (PLGA) particles loaded with purified protein/antigen. We also describe a method for generating bone marrow-derived DC in vitro. These DC can be subsequently used to measure the adjuvant properties of the particle formulations.
由可生物降解聚合物制备的基于颗粒的癌症疫苗是一种将抗原单独或与佐剂分子结合递送至树突状细胞（DC）的潜在有吸引力的方式。如果要启动有效的抗肿瘤免疫反应，非病毒疫苗接种模式除了体液反应外，还必须确保细胞免疫反应的激活。在这里，我们详细描述了一种制备负载纯化蛋白/抗原的聚（D，L-丙交酯-共-乙交酯）（PLGA）颗粒的方法。我们还描述了一种体外生成骨髓来源DC的方法。这些DC随后可用于测量颗粒制剂的佐剂特性。
