Kalantari Heibatullah, Galehdari Hamid, Zaree Zahra, Kalantar Hadi, Varnasary Golnaz
School of Pharmacy, Toxicology Research Center, Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
Jundishapur J Nat Pharm Prod. 2012 Winter;7(1):31-4. doi: 10.17795/jjnpp-3561. Epub 2012 Jan 4.
The increasing use of herbal drugs and their easy availability have necessitated the use of mutagenicity tests to analyze their toxicity and safety.
The aim of this study was to determine the in vitro mutagenicity of Artemisia draconculus L., a herbal drug, by performing single cell gel electrophoresis (SCGE).
In this study, we obtained a herbal drug with A. draconculus at a density of 0.94; doses of 100 μl, 200 μl, 400 μl, and 800 μl equivalent to 94 mg, 188 mg, 376 mg, and 752 mg of A. draconculus, respectively, were used. Sodium dichromate at a dose of 262 mg was considered to be the positive control, and blood was considered to be the negative control. Blood samples were centrifuged at 3500 rpm for 5 min, and the lower portion of the residue was isolated and mixed with low melting point agarose.
A cell suspension was prepared and applied on pre-coated agarose gel slides. Lysis, electrophoresis under alkaline conditions, staining of DNA, comet visualization, and comet scoring were carried out. The statistical analysis of the obtained results showed that with an increase in the dosage of A. draconculus, DNA damage also increased significantly (P < 0.05).
These findings provide valuable information regarding the safety and toxicity of this herbal drug, and this information will be helpful in ensuring rational use of this drug.
草药的使用日益增加且容易获取，因此有必要使用致突变性试验来分析其毒性和安全性。
本研究的目的是通过进行单细胞凝胶电泳（SCGE）来确定草药龙蒿的体外致突变性。
在本研究中，我们获得了密度为0.94的含龙蒿的草药；分别使用100μl、200μl、400μl和800μl的剂量，相当于94mg、188mg、376mg和752mg的龙蒿。262mg剂量的重铬酸钠被视为阳性对照，血液被视为阴性对照。血液样本以3500rpm离心5分钟，分离出下层残留物并与低熔点琼脂糖混合。
制备细胞悬液并应用于预先包被的琼脂糖凝胶载玻片上。进行细胞裂解、碱性条件下的电泳、DNA染色、彗星图像观察和彗星评分。对所得结果的统计分析表明，随着龙蒿剂量的增加，DNA损伤也显著增加（P<0.05）。
这些发现提供了有关这种草药安全性和毒性的有价值信息，这些信息将有助于确保该药物的合理使用。
